##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	Complete_Undetermined_L001_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14750073
Sequences flagged as poor quality	0
Sequence length	12
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.008307416512448	33.0	29.0	34.0	16.0	35.0
2	30.25339535607722	33.0	30.0	34.0	16.0	35.0
3	30.392043144464438	33.0	32.0	34.0	16.0	35.0
4	30.382115058006832	33.0	31.0	34.0	16.0	35.0
5	30.386181207374364	33.0	32.0	34.0	16.0	35.0
6	32.07513637390134	35.0	32.0	37.0	16.0	37.0
7	31.892406430802072	34.0	32.0	37.0	16.0	37.0
8	31.952718945865556	35.0	32.0	37.0	16.0	37.0
9	31.763630051186865	34.0	32.0	37.0	16.0	37.0
10	31.800210344721684	34.0	32.0	37.0	16.0	37.0
11	32.04130738878377	35.0	32.0	37.0	16.0	37.0
12	31.80931965557052	35.0	32.0	37.0	16.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2640051691958014
1101	2	0.25997708216252846
1101	3	0.25437677159279914
1101	4	0.17987634345615078
1101	5	0.2805439707325732
1101	6	0.343918160197326
1101	7	0.3342655746203107
1101	8	0.34141399761658064
1101	9	0.43529757731127816
1101	10	0.3112489255254012
1101	11	0.2801966758196812
1101	12	0.4164693901380012
1102	1	0.05638099468206903
1102	2	0.12264057164294329
1102	3	0.1432934577847682
1102	4	0.16265910071435385
1102	5	0.20013316319449714
1102	6	0.05488894633135999
1102	7	0.2207374517951095
1102	8	0.19415555454071765
1102	9	0.32271560970514557
1102	10	0.21250572329297412
1102	11	0.22043049181268515
1102	12	0.2602567715763122
1103	1	0.35200100839728776
1103	2	0.32393177499299597
1103	3	0.35023111416268193
1103	4	0.3269385187774496
1103	5	0.34386982172204483
1103	6	0.3729688247898366
1103	7	0.4248861845595826
1103	8	0.33723160674801633
1103	9	0.4331732204844734
1103	10	0.3597021411894836
1103	11	0.4357287497565068
1103	12	0.4388159796104212
2112	1	-0.13720148288142653
2112	2	-0.05875446751040059
2112	3	0.018347737041246148
2112	4	0.004157571750866396
2112	5	-0.05903252063803066
2112	6	0.07592441140487693
2112	7	0.1053704377930984
2112	8	0.027290127075712434
2112	9	0.05247164210883071
2112	10	0.053921618903551405
2112	11	0.1047790615400288
2112	12	0.13207734584422326
1113	1	0.16030452455552435
1113	2	0.15282501373245694
1113	3	0.16697249984126472
1113	4	0.1193296728893749
1113	5	0.19062072082285653
1113	6	0.2972909813408151
1113	7	0.3286003532821695
1113	8	0.08581190996079968
1113	9	0.1748899989460142
1113	10	0.036602029207543296
1113	11	0.1650957613195061
1113	12	0.37882087039637113
2113	1	0.21404547640437244
2113	2	0.23392844871223772
2113	3	0.19490736747613013
2113	4	0.242340388524088
2113	5	0.2501965440284195
2113	6	0.38224618289449097
2113	7	0.27391991636453383
2113	8	0.15471881452385006
2113	9	0.2844734978006507
2113	10	0.2430877587124094
2113	11	0.1713299495366698
2113	12	0.42781589884360827
1112	1	0.15042221126691402
1112	2	0.18117889929964548
1112	3	0.22773913592490302
1112	4	0.15557224626699195
1112	5	0.2599963260882596
1112	6	0.3583840532586109
1112	7	0.2143984971180828
1112	8	-0.0014558027680706687
1112	9	0.15448057796481862
1112	10	0.2014027908859184
1112	11	0.14950040251941488
1112	12	0.4137139254765039
2114	1	0.22931422563529225
2114	2	0.21957647715317208
2114	3	0.2627224796036849
2114	4	0.19666587614393194
2114	5	0.2622796548803663
2114	6	0.29114215721451586
2114	7	0.18403288118265948
2114	8	-0.01550518794292799
2114	9	0.19440727331155472
2114	10	0.21347564549208542
2114	11	0.14588894948961695
2114	12	0.378591182779342
1114	1	0.1987356453572886
1114	2	0.22870256191910343
1114	3	0.1582038056770756
1114	4	0.18060935032952585
1114	5	0.21336751271653753
1114	6	0.1884846481290552
1114	7	0.018029676948614792
1114	8	0.20885962596605268
1114	9	-0.06987571272572879
1114	10	-0.06961065867614025
1114	11	0.026262296751696113
1114	12	0.23710073784208774
1109	1	0.17719068658218973
1109	2	0.2511163983783504
1109	3	0.28250157206567295
1109	4	0.05750288062023046
1109	5	0.2138170840332343
1109	6	0.13967235667573874
1109	7	0.2518266811937764
1109	8	0.20795119124077743
1109	9	-0.20384367039552842
1109	10	-0.13535620114063107
1109	11	0.1753406235369468
1109	12	0.13850366537681325
1108	1	0.17577780869684645
1108	2	0.1688005747701844
1108	3	0.211238742761914
1108	4	0.04044737788838759
1108	5	0.09324206363531928
1108	6	0.17136850849642116
1108	7	0.2142590006571723
1108	8	0.1689301544202415
1108	9	-0.1591866667253079
1108	10	0.14100277543247586
1108	11	0.19507240848815144
1108	12	0.012438199822035756
1111	1	0.23224135326101347
1111	2	0.18428415863107972
1111	3	0.20124881326713862
1111	4	0.216055302625918
1111	5	0.22171122296840196
1111	6	0.2981828759507579
1111	7	0.3012509692256984
1111	8	0.2816559070254563
1111	9	0.22793264034370608
1111	10	0.30618002178306725
1111	11	0.18078969455645222
1111	12	0.07013531472819423
1110	1	0.12385635979466514
1110	2	0.06249918587201009
1110	3	-0.03131381917011211
1110	4	0.0525198787860468
1110	5	0.03570964949972932
1110	6	0.1519355083038363
1110	7	0.21710036153425705
1110	8	0.04003382880102535
1110	9	0.11925344402906646
1110	10	0.15990258714906602
1110	11	0.01610437646144902
1110	12	-0.13568305832318828
1105	1	-0.12840409037645628
1105	2	-0.04314064176329424
1105	3	-0.09405151425200486
1105	4	-0.04804875749598381
1105	5	-0.10856155981428728
1105	6	0.07452284979057566
1105	7	-0.05770941351308423
1105	8	-0.03967057836737453
1105	9	0.003755202689372794
1105	10	0.12528966556624965
1105	11	-0.20721313551586107
1105	12	-0.3895822551285235
1104	1	-0.01771674690133196
1104	2	0.04543307353115722
1104	3	-0.002048493936349871
1104	4	0.0741138365000289
1104	5	0.025492091768597902
1104	6	-0.04956739919446562
1104	7	0.12270927638076046
1104	8	0.1925527192022507
1104	9	0.24635638650542901
1104	10	0.13622994884900663
1104	11	0.17671655355039206
1104	12	0.06892906073362681
1107	1	-0.1972991674068183
1107	2	-0.12394594729881092
1107	3	-0.13900361271227268
1107	4	-0.0749665619536728
1107	5	-0.10915481066738764
1107	6	-0.3610140177548544
1107	7	-0.2274771075203752
1107	8	-0.0408540313310759
1107	9	0.08447101372828669
1107	10	-0.10321468762208497
1107	11	-0.06131149394750679
1107	12	-0.17238406995075906
1106	1	0.09200655036005756
1106	2	0.0556954770669158
1106	3	0.0452354752256241
1106	4	-0.09815075600410594
1106	5	0.01791630278309242
1106	6	0.006108074472301439
1106	7	0.09171399557772375
1106	8	0.15130180040165087
1106	9	0.23903453992197754
1106	10	0.055300541017647475
1106	11	0.1478764159801571
1106	12	0.10374918600729899
2103	1	0.1427419004645074
2103	2	-0.014425221370974128
2103	3	-0.06982017094670212
2103	4	0.05073340277537497
2103	5	0.0016129136102378538
2103	6	-0.011010461638342406
2103	7	-0.009754427050779668
2103	8	0.041314677974547465
2103	9	-0.28741068005239256
2103	10	-0.06502419652549918
2103	11	-0.018961871773235828
2103	12	-0.07905733162814599
2102	1	-0.011448410816541355
2102	2	-0.04621967798158266
2102	3	-0.04104705417111987
2102	4	-0.03902946902792692
2102	5	-0.017091688483930056
2102	6	-0.3052153211042423
2102	7	-0.2072490239232394
2102	8	-0.10206211009985822
2102	9	-0.3422627906273483
2102	10	-0.2250805303604082
2102	11	-0.12225514745808397
2102	12	0.13262187891830735
2101	1	-0.2142293955302783
2101	2	-0.09809954556538258
2101	3	-0.027128655103414445
2101	4	-0.1194819307788677
2101	5	-0.20507968535768484
2101	6	-0.15042940543570182
2101	7	-0.29399166110422925
2101	8	-0.11500449975198279
2101	9	-0.2986938443221341
2101	10	-0.1506376124300992
2101	11	-0.12311370421050682
2101	12	0.10026001756841296
2107	1	-0.001767133456862524
2107	2	-0.1062604928197679
2107	3	-0.09411727227739775
2107	4	-0.02583390602473301
2107	5	-0.13468593573114518
2107	6	-0.056989056553440776
2107	7	-0.08325407998211887
2107	8	-0.00821264001545785
2107	9	-0.19553296573059598
2107	10	-0.06391347877858067
2107	11	0.0474490233233098
2107	12	0.1291581464665441
2106	1	-0.08266444186621769
2106	2	-0.0635179701059414
2106	3	-0.0864451039166525
2106	4	-0.07096130051744254
2106	5	-0.09740074305009827
2106	6	-0.17919128991751165
2106	7	-0.3152264841147385
2106	8	-0.08722955317254844
2106	9	0.021763504772732034
2106	10	-0.10084172629960975
2106	11	-0.14795539375990785
2106	12	0.14276892437509048
2105	1	-0.29311017596699784
2105	2	-0.24797747398244496
2105	3	-0.28257082618238627
2105	4	-0.13671784757365657
2105	5	-0.18822002528485982
2105	6	-0.1516031108923137
2105	7	-0.17906765087606757
2105	8	-0.1929389095375491
2105	9	-0.11300910830225419
2105	10	-0.10840417322578233
2105	11	-0.08536192228361372
2105	12	-0.39951523021169777
2104	1	-0.11358174825107525
2104	2	-0.2070264019257948
2104	3	-0.25904143024572335
2104	4	-0.17227034952975018
2104	5	-0.2613230875767414
2104	6	-0.30192199587698454
2104	7	-0.4215348731228197
2104	8	-0.4305224457929704
2104	9	-0.30720697604096614
2104	10	-0.2703199921283108
2104	11	-0.18559229429131108
2104	12	-0.5771639565055189
2111	1	-0.27901422418969446
2111	2	-0.1993923753285216
2111	3	-0.218379388475352
2111	4	-0.07427347882841673
2111	5	-0.19707081936228832
2111	6	-0.40617924846498354
2111	7	-0.20141248522906352
2111	8	-0.30125149166414644
2111	9	-0.16442955211969945
2111	10	-0.13596741462850304
2111	11	-0.08443510021501055
2111	12	-0.534596724879421
2110	1	-0.3013899754457299
2110	2	-0.3691847017837482
2110	3	-0.3961377878132488
2110	4	-0.3231783591494377
2110	5	-0.3951731421233049
2110	6	-0.5559775282416091
2110	7	-0.5123720144590003
2110	8	-0.4042638903976794
2110	9	-0.3594714858201087
2110	10	-0.37565221744687705
2110	11	-0.3884621090151903
2110	12	-0.5427365781413585
2109	1	-0.3769373886698446
2109	2	-0.3933705555910656
2109	3	-0.29570397946429594
2109	4	-0.3557611276060122
2109	5	-0.32727997603488035
2109	6	-0.24538402736103393
2109	7	-0.26099041551691116
2109	8	-0.253110593022452
2109	9	-0.14060654667494887
2109	10	-0.3083870616654494
2109	11	-0.22046730626800937
2109	12	-0.32607744793959625
2108	1	-0.41425953289461503
2108	2	-0.5192742248368702
2108	3	-0.48020986375792774
2108	4	-0.5208479035588027
2108	5	-0.510435048359458
2108	6	-0.4325556768150278
2108	7	-0.5330616218209876
2108	8	-0.4411401816336422
2108	9	-0.35294613008637654
2108	10	-0.4434422220790317
2108	11	-0.9934319557044162
2108	12	-0.8254298437949217
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	58.0
15	4537.0
16	124499.0
17	250173.0
18	333616.0
19	397204.0
20	369482.0
21	395026.0
22	385999.0
23	337084.0
24	298238.0
25	279017.0
26	254342.0
27	242960.0
28	248162.0
29	259884.0
30	293504.0
31	369750.0
32	547222.0
33	1384301.0
34	3133873.0
35	4016629.0
36	824513.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.53569992501054	26.260188678388236	29.855133598321853	24.34897779827937
2	18.602992676714212	30.836850773552104	26.93039553092381	23.629761018809873
3	20.66652284815966	29.624994372908827	19.500847182305282	30.207635596626243
4	32.04270217022699	11.302237887684601	23.31176055435669	33.34329938773172
5	36.01604114203646	17.429277633356637	22.357199341128638	24.197481883478265
6	28.874277435779472	19.876789762328634	23.244691738135803	28.00424106375609
7	18.130297940240563	30.543762769429193	29.021892232119274	22.304047058210973
8	17.439066233773897	31.018863432065725	21.95865064532223	29.583419688838152
9	20.930432005319567	29.754422232350986	25.428674149612686	23.886471612716765
10	25.95566137198101	26.835277357610366	31.149981427210566	16.059079843198067
11	17.64980417385053	18.84928298320964	28.061664508372264	35.439248334567566
12	23.923645932033416	24.611764606979545	27.657173470068486	23.807415990918553
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4429.0
1	4429.0
2	4429.0
3	4429.0
4	11939.5
5	19450.0
6	19450.0
7	19450.0
8	19450.0
9	19450.0
10	19450.0
11	19450.0
12	19450.0
13	39795.0
14	60140.0
15	60140.0
16	60140.0
17	60140.0
18	60140.0
19	60140.0
20	60140.0
21	101963.5
22	143787.0
23	143787.0
24	143787.0
25	143787.0
26	143787.0
27	143787.0
28	143787.0
29	256853.5
30	369920.0
31	369920.0
32	369920.0
33	369920.0
34	369920.0
35	369920.0
36	369920.0
37	369920.0
38	2344430.0
39	4318940.0
40	4318940.0
41	4318940.0
42	4318940.0
43	4318940.0
44	4318940.0
45	4318940.0
46	4763504.5
47	5208069.0
48	5208069.0
49	5208069.0
50	5208069.0
51	5208069.0
52	5208069.0
53	5208069.0
54	4756774.5
55	4305480.0
56	4305480.0
57	4305480.0
58	4305480.0
59	4305480.0
60	4305480.0
61	4305480.0
62	4305480.0
63	2274584.0
64	243688.0
65	243688.0
66	243688.0
67	243688.0
68	243688.0
69	243688.0
70	243688.0
71	149704.5
72	55721.0
73	55721.0
74	55721.0
75	55721.0
76	55721.0
77	55721.0
78	55721.0
79	33967.0
80	12213.0
81	12213.0
82	12213.0
83	12213.0
84	12213.0
85	12213.0
86	12213.0
87	12213.0
88	7847.5
89	3482.0
90	3482.0
91	3482.0
92	3482.0
93	3482.0
94	3482.0
95	3482.0
96	4118.0
97	4754.0
98	4754.0
99	4754.0
100	4754.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.779627463538655E-6
4	2.0338882390615966E-5
5	6.779627463538655E-6
6	0.0
7	6.779627463538655E-6
8	0.0
9	0.0
10	0.0
11	0.0
12	6.779627463538655E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
12	1.4750073E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	5.399419093655059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.15277289149194	4.003818977977888
2	11.748497068589295	1.2687011878778303
3	4.478206732736321	0.7253914481421337
4	2.4014194414073144	0.5186507993523647
5	1.217798875454246	0.3287703250179657
6	1.000423427766244	0.32410232145725404
7	0.6995569368165792	0.26440407572223973
8	0.5351043345708966	0.23114020489437467
9	0.392261853571728	0.19061875276989415
>10	2.8468886590514	2.9253220113334715
>50	0.23115930754211644	0.8801565159287138
>100	0.22494180565130603	2.741928194725321
>500	0.048809949546900454	1.8040125645683776
>1k	0.016163377325809667	1.4845055781554346
>5k	0.0	0.0
>10k+	0.005995338477970263	82.30847704207667
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTCGTTCGACT	514836	3.4903962848183867	No Hit
CACGCCATAATG	493993	3.349088509595851	No Hit
TTAGGGCTCGTA	458513	3.1085473271894997	No Hit
TCCGACACAATT	340641	2.309419078807271	No Hit
GCACACACGTTA	326555	2.2139212463558655	No Hit
CTGCTAACGCAA	319616	2.1668774113863707	No Hit
ATGTGCACGACT	310053	2.1020438339525507	No Hit
TCACGGGAGTTG	308401	2.0908438893827848	No Hit
CAGGGTGATACC	307002	2.0813591905612943	No Hit
ATCCCGAATTTG	305501	2.0711829697385227	No Hit
ACGCGCAGATAC	303444	2.0572372760460236	No Hit
CCAGTGTATGCA	295649	2.00439007996774	No Hit
TACACGATCTAC	288217	1.9540038886587205	No Hit
GTTGGTCAATCT	285975	1.938803963885467	No Hit
TAGCTCGTAACT	277803	1.883400848253429	No Hit
CTAACCTCCGCT	275846	1.870133117307284	No Hit
GACTTGGTATTC	271112	1.8380383608948918	No Hit
GGATGGTGTTGC	259830	1.7615506038512487	No Hit
TAGGATTGCTCG	259314	1.7580523160800627	No Hit
TTACTGTGCGAT	244811	1.6597273789763618	No Hit
TATCGTTGACCA	236978	1.6066225570544632	No Hit
GACTTTCCCTCG	234606	1.5905412807109498	No Hit
GGATCGCAGATC	228458	1.548860131065114	No Hit
AGTTCAGACGCT	225264	1.5272060009465716	No Hit
CAGGCGTATTGG	221091	1.4989146155412247	No Hit
AGCAGTCGCGAT	220664	1.4960197146142937	No Hit
GCTGATGAGCTG	220207	1.4929214248634566	No Hit
CTTATCAAGGCG	216643	1.468758832583405	No Hit
AACGGATAAACG	216556	1.468169004994077	No Hit
ATTTCGACATGC	215986	1.46430461733986	No Hit
ACACCTGGTGAT	215807	1.4630910640238866	No Hit
TGAGAACCTCCA	215725	1.4625351345718764	No Hit
AGCTGTTGTTTG	214470	1.4540267021051354	No Hit
TTCAGACTACCA	213666	1.4485758816244503	No Hit
TGCCTACAAGCA	201210	1.3641288419386128	No Hit
AATCAGTCTCGT	200407	1.3586848010853911	No Hit
GAGTGAGTACAA	199457	1.3522441549950295	No Hit
AATCCGTACAGC	195365	1.3245019194142293	No Hit
GGAGACAAGGGA	192878	1.3076409859124087	No Hit
ATCGAATGCGGC	190816	1.293661394082592	No Hit
CAGTGCATATGC	189974	1.2879529477582925	No Hit
TGAGTCACTGGT	189190	1.2826377198268781	No Hit
GTATGCGCTGTA	185644	1.2585971608411701	No Hit
TGCCCATTTAAG	176809	1.1986991522008061	No Hit
ACCGTGCATAGG	169849	1.151512945054577	No Hit
AGGCTACACGAC	159691	1.0826454892799515	No Hit
TAATGACCACGC	155856	1.0566456179572807	No Hit
>>END_MODULE
>>Adapter Content	warn
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCCG	80	6.539255E-5	6.0000005	4
TAGCGCG	80	6.539255E-5	6.0000005	1
TAACGGG	80	6.539255E-5	6.0000005	1
TAGCCCG	80	6.539255E-5	6.0000005	1
TCAATGG	80	6.539255E-5	6.0000005	5
TACGCCA	235	0.0	6.0000005	1
CGGGCAG	80	6.539255E-5	6.0000005	2
TGAGGCA	80	6.539255E-5	6.0000005	1
GGCTGGC	95	3.4297518E-6	6.0000005	1
GCGTCTT	160	1.0913936E-11	6.0000005	4
AATGTGG	80	6.539255E-5	6.0000005	5
GAGGCAC	80	6.539255E-5	6.0000005	2
TCCGGAA	55	0.009160625	6.0	2
ATGGGAG	110	1.8152969E-7	6.0	3
ACTGCAT	155	2.7284841E-11	6.0	2
CATATGG	60	0.0033965306	6.0	6
GGGTCAT	60	0.0033965306	6.0	3
TGTGGGA	60	0.0033965306	6.0	5
CGGTTAA	110	1.8152969E-7	6.0	3
GGCGTCT	130	3.6470738E-9	6.0	3
>>END_MODULE