FastQCFastQC Report
Thu 21 Aug 2014
Complete_Undetermined_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameComplete_Undetermined_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14750073
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGTTCGACT5148363.4903962848183867No Hit
CACGCCATAATG4939933.349088509595851No Hit
TTAGGGCTCGTA4585133.1085473271894997No Hit
TCCGACACAATT3406412.309419078807271No Hit
GCACACACGTTA3265552.2139212463558655No Hit
CTGCTAACGCAA3196162.1668774113863707No Hit
ATGTGCACGACT3100532.1020438339525507No Hit
TCACGGGAGTTG3084012.0908438893827848No Hit
CAGGGTGATACC3070022.0813591905612943No Hit
ATCCCGAATTTG3055012.0711829697385227No Hit
ACGCGCAGATAC3034442.0572372760460236No Hit
CCAGTGTATGCA2956492.00439007996774No Hit
TACACGATCTAC2882171.9540038886587205No Hit
GTTGGTCAATCT2859751.938803963885467No Hit
TAGCTCGTAACT2778031.883400848253429No Hit
CTAACCTCCGCT2758461.870133117307284No Hit
GACTTGGTATTC2711121.8380383608948918No Hit
GGATGGTGTTGC2598301.7615506038512487No Hit
TAGGATTGCTCG2593141.7580523160800627No Hit
TTACTGTGCGAT2448111.6597273789763618No Hit
TATCGTTGACCA2369781.6066225570544632No Hit
GACTTTCCCTCG2346061.5905412807109498No Hit
GGATCGCAGATC2284581.548860131065114No Hit
AGTTCAGACGCT2252641.5272060009465716No Hit
CAGGCGTATTGG2210911.4989146155412247No Hit
AGCAGTCGCGAT2206641.4960197146142937No Hit
GCTGATGAGCTG2202071.4929214248634566No Hit
CTTATCAAGGCG2166431.468758832583405No Hit
AACGGATAAACG2165561.468169004994077No Hit
ATTTCGACATGC2159861.46430461733986No Hit
ACACCTGGTGAT2158071.4630910640238866No Hit
TGAGAACCTCCA2157251.4625351345718764No Hit
AGCTGTTGTTTG2144701.4540267021051354No Hit
TTCAGACTACCA2136661.4485758816244503No Hit
TGCCTACAAGCA2012101.3641288419386128No Hit
AATCAGTCTCGT2004071.3586848010853911No Hit
GAGTGAGTACAA1994571.3522441549950295No Hit
AATCCGTACAGC1953651.3245019194142293No Hit
GGAGACAAGGGA1928781.3076409859124087No Hit
ATCGAATGCGGC1908161.293661394082592No Hit
CAGTGCATATGC1899741.2879529477582925No Hit
TGAGTCACTGGT1891901.2826377198268781No Hit
GTATGCGCTGTA1856441.2585971608411701No Hit
TGCCCATTTAAG1768091.1986991522008061No Hit
ACCGTGCATAGG1698491.151512945054577No Hit
AGGCTACACGAC1596911.0826454892799515No Hit
TAATGACCACGC1558561.0566456179572807No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG806.539255E-56.00000054
TAGCGCG806.539255E-56.00000051
TAACGGG806.539255E-56.00000051
TAGCCCG806.539255E-56.00000051
TCAATGG806.539255E-56.00000055
TACGCCA2350.06.00000051
CGGGCAG806.539255E-56.00000052
TGAGGCA806.539255E-56.00000051
GGCTGGC953.4297518E-66.00000051
GCGTCTT1601.0913936E-116.00000054
AATGTGG806.539255E-56.00000055
GAGGCAC806.539255E-56.00000052
TCCGGAA550.0091606256.02
ATGGGAG1101.8152969E-76.03
ACTGCAT1552.7284841E-116.02
CATATGG600.00339653066.06
GGGTCAT600.00339653066.03
TGTGGGA600.00339653066.05
CGGTTAA1101.8152969E-76.03
GGCGTCT1303.6470738E-96.03
Produced by FastQC (version 0.11.2)