FastQCFastQC Report
Thu 21 Aug 2014
Complete_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameComplete_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14750073
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACTACCGATTGAATGGCTTAGTGAGCCTTTGGGATTGGCAGCTTGTA210473414.269312429843566No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGG8195845.5564741950768655No Hit
GCTACTACCGATTGAATGGCTTAGTGAGATCTCCGGATTGGCTTTGGGAA5195043.522043585818185No Hit
GCTCCTACCGATTGAATGATCCGGTGAAGTGTTCGGATTGCGTCGACGAC2738701.8567365734393315No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCCTTTGGGATCGGCAGCTTGTA2697591.828865524936724No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGTG2632421.7846826927568429No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGGG2539921.7219711387191101No Hit
GCTACTACCGATTGAACGGCTTGGTGAGATCTCTGGATTGGCGTTGGGGA1865751.2649089940097245No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTCCTCG1683381.1412689279571702No Hit
GCTACTACCGATTGAATTGTTTAGTGAGGTCTCCGGACGTGATCGCTGCG1666781.130014746367696No Hit
ACACCATGGGAGTGGAGGATACCAGAAGTAGTTAGGCTAACCGCAAGGGG1456150.9872154531031814No Hit
GCTCCTACCGATTGAATGATCCGGTGAAATGTTCGGATCGCGGCGACGTG1261540.8552771230352555No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCCTTTGGGACTAGCCGCTTGTA1179590.7997180759715563No Hit
GCTCCTACCGATTGAGTGATCCGGTGAATTTTCTGGACTTCTGGTTCATG1046490.7094812344318567No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGGCTATTGGGA955540.6478205226509727No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG934210.6333595772712447No Hit
GCTACTACCGATTGAATGGCTGAGTGAGGCCTTCGGACTGGCCCAGGGAG804650.5455227238536379No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCGAGGAG725610.4919365483818283No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCGGAGAG705810.4785128860040218No Hit
GCTCCTACCGATTGAATGATCCGGTGAAGTGTTCGGATCGCGGCGACGTG699080.47395019672106026No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTGGATC568180.3852048732233393No Hit
GCTACTACCGATTGGATGGCTTAGTGAGGCCCTCGGATCGGCCCCGCCGG553090.37497441538085946No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGGCTTAAGAAA540650.3665405588162174No Hit
GCTCCTACCGATTTCGAGTGATGTGGTGAACTATCTGGACTGCGGATGGG513760.34831014056676196No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTTGGATGGTGGCGATAGA492480.3338830933243517No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTCTTAT489520.33187632359514424No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATTGCGGCGACGTG472980.3206628197704513No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTCCGGACTGGCTCGAAGAG454560.3081747459826131No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTCAGGATTGGCTTATGGCG434230.29439176334923906No Hit
GCTCCTACCGATTGGGTGTGCTGGTGAAGCGTTCGGATTGGCGTTAGTGG412640.2797545476554591No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG411970.27930031261540195No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTGACTT410560.278344385143043No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTTTAGG409200.2774223558080018No Hit
GCTACTACCGATTGGAAGTTTTAGTGAGGCATTTGGACCTGCCATCTAAA404310.27410711797833137No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCG401210.27200543346463435No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG367310.2490224963632383No Hit
GCTCCTACCGATTGAATGATCCGGTGAAGTGTTCGGATCGCGTCAACGAG364290.24697504886924967No Hit
GCTCCTACCGATTGGATGACTCGGTGAAATCACCGGATGCTTACGAGCAG364030.24679877855519763No Hit
TTCTGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTCTTATCAAGG359090.24344964258820956No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTCAAGATTGGCGCCGCGAT346450.23488019347429673No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTCCGGATTGGTTTAGAGAA344480.23354460686397957No Hit
GCTACTACCGATTGGAAGTTTTAGTGAGGCATTTGGACCTGCTCTTTAGG330990.22439888941566594No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTTATCG329810.22359889337496838No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTGGGATTGGCTTAGGAGG323610.2193955243475744No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGGTTTAAAGAA320530.2173073990888045No Hit
GCTACTACCGATCGGATGGTTTAGTGAGGTCCTCGGATCGGCCCCGCCGG315870.2141480926907955No Hit
GCTACTACCGATTGGAAGTTTTAGTGAGGCATCTGGACTTGCTGGCCAGG310580.21056166976258353No Hit
GCTACTACCGATTGAATGGCTCGGTGAGGCCTTCGGACTGGCCCAGGGAG310030.21018879025208895No Hit
GCTACTACCGATTGAACGGCTCAGTGAGGCCTTCGGACTGGCTCAGGGAG309040.2095176071331986No Hit
GCTCCTACCGATACCGGGTGATCCGGTGAACCTTTTGGACCGTTCGCGGA306970.2081142242482461No Hit
GCTCCTACCGATTTCGAGTGATCCGGTGAACCATTTGGACCGCGGAGTTC294370.19957189364418737No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTTCGGATTGGCTTTTGGGA286360.19414141204589294No Hit
GCTACTACCGATCGGATGGTTTGGTGAGGTCCTCGGATCGGCCCCGCCGG285540.19358548259388275No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGAGGCGACGGG273260.18526010006865729No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTGGGATTGGCCAGGGGAG273010.1850906093820688No Hit
GCTCCTACCGATTGAATGATCCGGTGAAGTGTTCGGATTGCGTCGACGAT271290.18392451345834018No Hit
GCTACTACCGATTGAATGGTTTAGTGAGATCATCTGAGCAGATGTGCATG270040.18307706002539784No Hit
GCTGCTACCGATTGAATAGTAAATGTGAAATGGGAGGACTATCGTCTGAA269610.18278553604446568No Hit
GCACCTACCGATTGAATGATTCGATGAATTTTTCGGACTGTGATCAGGAC258040.17494150706915146No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTAGGATCGCGGCGACGTG248710.1686161146456699No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGTCCTCGGATCGGCCCCGGCGG242690.1645347789126196No Hit
GCTACTACCGATTGAATGTTTTAGTGAGATCTTCGGCTTGGTTTTCCGAG241740.16389071430358346No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTTGGGATTGGCTTCGGGGA237220.16082632269006397No Hit
GCTACTACCGATTGAATGGCTTAGTGAGACCTCCGGATTGTCGTTGGGCT228900.1551856726403998No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGGCTTCGGGGA227460.15420940628565025No Hit
TCTACTACTGATAGGATGGTTTAGTGAGGTCCTCGGATCGGCCCCGCCGG222750.15101620175032354No Hit
GCTACTACCGATTGAATGGCTTAGTGAGACCTCCGGATTGGCTTCGGGGA221380.15008739278781874No Hit
ACACCATGGGAATTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG220080.14920604121755873No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTCAAGATTGGCGCCGCGGG214920.14570775344637277No Hit
TTCTGCTTGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTCCTCGT204820.13886032970819873No Hit
GCTACTACCGATTGAATGGCTAAGTGAGGCTTTCGGACTGGCCTAGGGAG198600.1346434014258777No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCCTTTGGGATTGGCAGCTTGGA197580.13395187942459674No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCCCTTGGGATTGGCAGCTTGTA190930.12944342716134355No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCGGGGAG188830.12801970539400043No Hit
ACTACTACCGATTGAATGGCTTAGTGAGGCTTCCGGATTGGTTTAAAGAA188780.12798580725668274No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTCCGGATTGGTTTAGGAAA187240.12694174462729776No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCCTTTGGGATTGGCCGCTTGTA185030.12544344695785573No Hit
GCTCCTACCGATTGGATGACTCGGTGAAATGACTGGACTATTGGAAGGTC181580.12310447548293489No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCCTTTGGGATCGGCAGCTTGGA177880.12059601332142558No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGAGGCGACGTG175190.11877229353373368No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTTCGGATTGGCTTTGGGGA174730.11846043067041093No Hit
GCTACTACCGATTGGAAGTTTTAGTGAGGCATCTGGACTTGCCGTCCACG174660.11841297327816615No Hit
GCTACAACCGATTGAATGGCTTAGTGAGGCCTTCGGACTGTCCCAGGGAG173340.11751806245297904No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCCTTTGGGACTGGCAGCTTGTA173080.11734179213892704No Hit
ACACCATGGGAGTGGGGGATACCAGAAGTAGGTAGGGTAACCGCAAGGAG170950.11589773148919331No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCAGACTGGCTAATTCGA164530.1115452106576015No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCAGGGAG163460.11081979051900286No Hit
GCTATTACCGATTGGGTGTATAGGTGAGCCTTCTCGATAGACGCAAGTCT160910.10909098551580049No Hit
GCTACTACCGATTGAACGGCTTAGTGAGACCTCCGGATTGAATAGTCGGG159870.10838590425959248No Hit
ACACCATGGGAGTGGGTTGTACCAGAAGTAGATAGCTTAACCTTTTGGAG159810.10834522649481124No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTTGGGATTGGCTTCGGGGA156840.10633167713814026No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCCTTCGGGATTGGCAGCTTGTA150070.1017418693453246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACC8493550.094.986583
CTACTAC8503500.094.986042
CCGATTG10753550.094.948328
CGATTGA9062500.094.939729
ACCGATT10875450.094.899997
CTACCGA10969950.094.865775
GCTACTA8408550.094.8644261
TACCGAT11025000.094.806566
TCCTACC2264750.094.804953
ACTACCG8511900.094.804694
CTCCTAC2271650.094.79092
CGATTGG1701600.094.734819
GCTCCTA2271550.094.3287661
CCTACCG2384350.094.300764
ACCATGG736650.094.290723
CATGGGA755050.094.1570055
ACCGATC130150.093.97817
GTTACTA25350.093.688361
CACCATG756100.093.523682
CCGATTT135500.093.492638
Produced by FastQC (version 0.11.2)