FastQCFastQC Report
Mon 5 Aug 2024
AA05_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA05_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9500742
Sequences flagged as poor quality0
Sequence length1-93455
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACC429520.4520910051025488No Hit
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATAC282110.29693470257375687No Hit
ATGTCCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCAC269670.28384098841964134No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACC254920.26831588522243843No Hit
TTGTCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACC222710.23441327003722445No Hit
ATGTCCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATA194260.20446824048058562No Hit
TTGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATAC185220.19495319418209653No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACC152390.16039799838791538No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATAC151610.1595770098798599No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCA144400.15198812892719327No Hit
TTGCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATACG134810.14189418047558813No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACCG132720.13969435229374716No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACCGAGAT130690.13755767707406433Illumina Single End PCR Primer 1 (100% over 23bp)
TTGCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACCG126880.1335474639770241No Hit
ATGTACGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACCG118730.12496918661721369No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCAT117290.12345351552541896No Hit
ATGTACCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCAC116050.12214835430748461No Hit
TTGCCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACC110110.11589621105383137No Hit
TATGTCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCAC102040.1074021376435651No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATACGAGAT101220.10653904716073756Illumina Paired End PCR Primer 2 (95% over 24bp)
TTTGTCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCAC100420.10569700766529604No Hit
TTTGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATA99930.10518125847433811No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATACG99740.10498127409417075No Hit
TTTGCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATAC98660.10384452077532472No Hit
ATGTCCTCTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACC96890.10198150839166036No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATAC95500.10051846476833073No Hit
GTGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATAC95480.10049741378094469No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)