FastQCFastQC Report
Tue 30 Jul 2024
AA03_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA03_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114
Sequences flagged as poor quality0
Sequence length82-16213
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG43.508771929824561No Hit
GTGTACGTACTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATACGA10.8771929824561403Illumina Single End Adapter 1 (95% over 21bp)
TTTGTAGCCTCTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATAC10.8771929824561403No Hit
GTTATGCCTGCTTCGACTCATCTCGTGTGCTACAGGCGACCACCGAGATC10.8771929824561403No Hit
ATGTTTATGTACGTGTTACTTGGTTCAGTTACGTATTGCTAAGCAGAAGA10.8771929824561403No Hit
TTGTTAACCCTACTTCGTTCAGTTACGTATTGCTAAGCAGAAAACAGCAT10.8771929824561403No Hit
TGTTATGTGCTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATACG10.8771929824561403No Hit
TTTGTCCTGTTACTTCGTTCAGTTACGTATTGCTAATGATTCCGGCGACC10.8771929824561403No Hit
TTTGGCATGTACTTGGTTCAGTTACGTATTGCTAGCAGAAGACGGCATAC10.8771929824561403No Hit
GTTATGTCATTATTTACTTGGTTCAGTACGTATTGCTGCATACGAGATAA10.8771929824561403No Hit
ATGTTTTTTTTACTTGGTTCAGTTGGTCTTGCTAAGCAAAGCAGCCGTCG10.8771929824561403No Hit
GTGCCTGTTACTCGTTCAGTTTGGTCTTGCTTTTTACTCCATTTGCTCCA10.8771929824561403No Hit
GTGCCTGTTACTTGGTTCAGTTCGTATTGCTAAGCAGAAGACGGCATACG10.8771929824561403No Hit
TTGTTGGCACCTGGTTCAGTTACGTATTGCTAAGCAAAGACGGCGTCAGG10.8771929824561403No Hit
ATGTCGTACTTGGTTCAGTTGCGTATTGCTAATAATACGGCGACCACCGA10.8771929824561403No Hit
GTTATGCCTGCTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACCG10.8771929824561403No Hit
ATGTGTGACCTACTGGTTCGGTTGGTCTTGCTAATGATACGGCAGCACCG10.8771929824561403No Hit
ATGTTGTTGGTTCTTGGTTCAGTTACGTATTGCTAAGCAGAAGACGGCAT10.8771929824561403No Hit
ACTTTTGTGTGCTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCAC10.8771929824561403No Hit
GTGCATCTTACTTCGTTCCATTCTAGGTCTTGCTAAGCAGAAGACGGCAT10.8771929824561403No Hit
GTTATGTTAACCTACTTGGTTCAGTTACGTATTGCTAAGCAGAAGACCGG10.8771929824561403No Hit
GTGTCATTTTTTAACAACATTTTAGCAAAAACAGCATACGAGATGAGAAT10.8771929824561403No Hit
GTGTAGGTTACTTGGTTCAGTTACGTATTGCTAATGATACGGCGACCACC10.8771929824561403No Hit
ATGTCCTCTACTCGTTCAGTTGCGTATTGCTTGGCGAAAGACGGCATTAC10.8771929824561403No Hit
ACTGTCCGTTTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCA10.8771929824561403No Hit
TATGTTATGTAGTCTATTGGTTAGAGTACGTATTGCTAAGCAGAAGACGG10.8771929824561403No Hit
TATGTGCCTGTGCTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCAT10.8771929824561403No Hit
ATGTCCCTATTTACTGGTTCCAGTACGTATTGCTAATGATACGGCGACCA10.8771929824561403No Hit
TATGTACATCTACTTCGTTCAGTTACGTATTGCTAATGATACAGCAACCA10.8771929824561403No Hit
ATGTAGCCTACTCGTTCAGTTACGTATTGCTAATGATACAGCAACCGCTA10.8771929824561403No Hit
GTCCCTCTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATACG10.8771929824561403No Hit
ATGTCCTGTTACTTGGTTCAGTTACGTATTGCTAATGATACGGCGACCAC10.8771929824561403No Hit
TTTGCCATGCTTCAACATTCGTGCTCACGGAAGAACGACGGTCCGCGATA10.8771929824561403No Hit
ATGTTAACTTACTCGTAGGATTACGTATTGCTAATGATACGGCGACCACG10.8771929824561403No Hit
GTTTTGTAGCTTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACC10.8771929824561403No Hit
TGTTGTGTATATACTGGTTCAGTTACGTATTGCTAAGCAAGAGCAGCAGT10.8771929824561403No Hit
ATGTGACGTGTGTAAGATTACGTATTGTTAATGAATACAGCAACCACCGA10.8771929824561403No Hit
GTTGTGGTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCAC10.8771929824561403No Hit
ATGTACTTGGTTCGGACGCGTATTGCTCAGCGAACGGCATGCGATGAGAT10.8771929824561403No Hit
GTGTTATGTGTCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACAGC10.8771929824561403No Hit
ATGTTGTGTAGCATCTCGTTCGTTGGTCTTGCTAGCAGAAGACGGCATAC10.8771929824561403No Hit
GTTATGTTTATCTACTTCGTTCAGTTACGTATTGCTAGCAGAAGACGGCA10.8771929824561403No Hit
TTGTTGCTTGGTTCAGTTACGTATTGCTTAAGCAGAAGACGGCATACGAG10.8771929824561403Illumina Single End Adapter 1 (95% over 22bp)
ATGTTTATGTTTGACCCTTCTTCGTTCAGTTACGTATTGCTAAGCAGAAG10.8771929824561403No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGAGAGAGA10.8771929824561403No Hit
TTATGTTGGTTACTCGTTCAGTTACGTATTGCTAAGCAGAAAGACGGCAT10.8771929824561403No Hit
GTTATCTGCATATTTACTTCGTTCAGTTACGTATTGCTAATGATACGGCG10.8771929824561403No Hit
GTGTTATGTAGCACTTGGTTCAGTTACGTATTGCTAATGATACGGCGACC10.8771929824561403No Hit
GTGTTATGTACATCTACTTCGTACAGTTACGTATTGCTAATGATACAGGC10.8771929824561403No Hit
ACTGTTAGCCTTGGTTCAGTTAGCTGCTAAGCAGAAGACAGCATCCGAAT10.8771929824561403No Hit
GTTGTGCCTATACCTTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGC10.8771929824561403No Hit
ATGTTTATGTTAACCTACTCGTTCAGTTACGTATTGCTAGCAGAAGACGG10.8771929824561403No Hit
GTGTGTGCTTGGTTCAGTTACGTATTGCTAATGATACGGCGACCACCGAG10.8771929824561403Illumina Single End PCR Primer 1 (100% over 21bp)
TGTGTAACCTACTCATTCATCATATGCTAATGATACGGCGACCACCGAGA10.8771929824561403Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATATATATATATATATATATATATATATATAT10.8771929824561403No Hit
ATGTACAACAGCTTGTACCTCGTTCAGTTACGTATTGCTAAGCAGAAGAC10.8771929824561403No Hit
ATGTTGTGCGTATTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCA10.8771929824561403No Hit
GTTATGTTGTGTGTATTTCGTTCAGTTACGTATTGCTAATGATACGGCGA10.8771929824561403No Hit
GTGTTATGTACGTGTACCTCGTTCAGTTACGTATTGCTAAGCAGAAGACG10.8771929824561403No Hit
ATTGCTCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCA10.8771929824561403No Hit
TGTGCCTGTACTTCGTTCAGTTACGTATTGCTAATAATTAGGCGACCACC10.8771929824561403No Hit
GTTATAACTTACTTGGTTGGTGACATTGCTCACAAAGCAGCCGTCGATGA10.8771929824561403No Hit
ATGTTATGTCCGTATATTCGTTCAGTTACGTATTGCTAGCAGAAGACGGC10.8771929824561403No Hit
GTGTAACCTACTCGTTCAGTTGCGTATTGCTAATGATACGGCGACCGCCA10.8771929824561403No Hit
ATGTTATGTAACTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATAC10.8771929824561403No Hit
GTGCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGGCGACCACC10.8771929824561403No Hit
ATTGTCCTACTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACGAG10.8771929824561403No Hit
GTGTGGTGTGCTCGGTTCAGTTACGTATTGCTAGCAGAAGACGGCATACG10.8771929824561403No Hit
ATGTCACTTACTTGGTTCAGTTTCGTATTGCTAAGCAGGAAGAAGCGGCA10.8771929824561403No Hit
TGTTATGTTAGCCTTCGACATTACGTATTGCTAATGATACGGCGACCACC10.8771929824561403No Hit
TTTTTTTGCCACTTACTTGGTTCAGTTACGTATTGCTAGCAGAAGACGGC10.8771929824561403No Hit
ATGTTTTGTTCCTTGAGCTTTCAGTTACATATGCTCTCTTTACCTACTGC10.8771929824561403No Hit
ATGTTCACGCCTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACC10.8771929824561403No Hit
GTTATGTTAGTATTTATTGACTAGATTACGTATTGCTAATGATACGGCGA10.8771929824561403No Hit
TTGTTGGCCTGTACTTGGTTCAGTTACGTATTGCTAATGATACGGCGACC10.8771929824561403No Hit
CTGTCCCTGTTCTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATA10.8771929824561403No Hit
ATATGTTAGTCTGCTTCGTTCAGTTACGTATTGCTAAGCAGAAAGACGGG10.8771929824561403No Hit
CTGTGCTGCTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACCGAG10.8771929824561403Illumina Single End PCR Primer 1 (100% over 21bp)
TTTGCCCTCTACTTGGTTCAGTTACGTATTGCTAATGATACGGCGACCAC10.8771929824561403No Hit
ATATGTGCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCAT10.8771929824561403No Hit
GTGTGGCTGGACCGATTACGTATTGCTAATGATACGGCGACCACCGAGAT10.8771929824561403Illumina Single End PCR Primer 1 (100% over 23bp)
AGCCTGGCCAGCCTGGCTTTGAAAAAAAAAAAAAAAAAAAAAAAAGAGAG10.8771929824561403No Hit
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATAC10.8771929824561403No Hit
ATGTTGTGTCCTCTACTTGGTTCAGTTACGTATTGCTAATGATACGGCGA10.8771929824561403No Hit
GTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATACGAGAT10.8771929824561403Illumina Paired End PCR Primer 2 (95% over 24bp)
CTGTGCCTGTACTACGTACGTACGTATTGCTAATGATACGGCGACCACCG10.8771929824561403No Hit
TGTACACGTCTTTTTGGTTCACAAGTAGTATTGCTAACAGAGACGGCATA10.8771929824561403No Hit
TTATGTTAGTTGTTCTTTGGTTCAGTTACGTATTGCTAAGCAGAAGACGA10.8771929824561403No Hit
ATTTTGCCACCTTGGTTCAGATTACGTATTGCTAAGCAGAAGACGGCATA10.8771929824561403No Hit
TGGCCCTCCTCTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATAC10.8771929824561403No Hit
TATGTCGCTTATTTACTGGTTCAGTTACGTATTGCTAATGATACGGCGAC10.8771929824561403No Hit
TTTTTTGTTGGTCTATTCGTTCAGTTACGTATTGCTAATGATACGGCGAC10.8771929824561403No Hit
TGTTTATGTGTAACCTACTCGTTCAGTTACGTATTGCTAATGATACGGCG10.8771929824561403No Hit
ATATGTCTTTTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCAT10.8771929824561403No Hit
TTTGCCCTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATACG10.8771929824561403No Hit
GTGCCTGCTACTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACCG10.8771929824561403No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAGCGGAAGAAGCGGCA10.8771929824561403No Hit
TTATGTGCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCA10.8771929824561403No Hit
TTATGTTGGTTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGAC10.8771929824561403No Hit
ATCTGGCGTACCTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACC10.8771929824561403No Hit
TTTGCCGTCTACTCGTTCAGTTTCGTATTGCTCAGCGGAAGAACAGCATA10.8771929824561403No Hit
GTGTATGTGGTGTACTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCA10.8771929824561403No Hit
GTATTGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAAGACGGC10.8771929824561403No Hit
TATGTGCCTGTTGCTTCGTTCAGTTACGTATTGCTAATGATTGGGCGACC10.8771929824561403No Hit
GTGTAACCTACTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATCAA10.8771929824561403No Hit
ATGTGTATATTTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACC10.8771929824561403No Hit
GTGTAGCACCTCGTTCGTTCGTATTGCTAATGATACGGCGACCACCGAGA10.8771929824561403Illumina Single End PCR Primer 1 (100% over 22bp)
TATGTACCTTTACTGGTTCAGTTACGTATTGCTAATGATACAGCAACCAC10.8771929824561403No Hit
AGCCTACTTGGTTGAGAATTCTCGTATTGCTAAGCAGAAGACGGCATCAG10.8771929824561403No Hit
TTATGTTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAAGAAGACGG10.8771929824561403No Hit
ATGTAACCTACTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATACG10.8771929824561403No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)