FastQCFastQC Report
Tue 30 Jul 2024
AA03_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA03_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length43-3504
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG37.5No Hit
TGTTTTGTAACCCTACTCGTTCAGTTACGTATTGCTAATGATACGGCAAC12.5No Hit
TATGTTTATGTACCTACTCGTTCAGTTACGTATTGCTAAGCAGAAGACGG12.5No Hit
AGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA12.5No Hit
GTGTTGTGTGGGCGTGCCTACTCGTTCAGTTACGTATTGCTAAGCAGAAG12.5No Hit
ATGTCGTCCTTGACTCGTCGTCTTGCTAAGCAGAAGACGGCATACGAGAT12.5Illumina Paired End PCR Primer 2 (95% over 24bp)
GCTGTGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAAAAACGGCAT12.5No Hit
GTTATGTTATTGTACTTGGTTCAGTTCCATGCTTTGCTATAATTAGGCAA12.5No Hit
ATGTTGTGTCCTGTTACTTCGTTCAGTTACGTATTGCTAATGATACGGCG12.5No Hit
ATATGTAACCTACTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCCAT12.5No Hit
TGCCTGTTCTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACCGAG12.5Illumina Single End PCR Primer 1 (100% over 21bp)
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATAC12.5No Hit
GTGTTATCTAGTTACTTGGTTCCGTTGGTCTTGCTATGCTCTGCAGCAAC12.5No Hit
GTGCCTGTTACTTGGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATAC12.5No Hit
TGTTTTGTTATGTACATATTACTTGGTTAGAATTCTCGTATTGCTAATGA12.5No Hit
GTTGTGCCCTGTACTTCGTTCAGTTACGTATTGCTAATGATACGGCGACC12.5No Hit
ACTTTTTTGCCTGTACTTGGTTCAGTTAGCGTATTGCTAAGCAGAAAAAC12.5No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTCTCTCTCTCTGTG12.5No Hit
CATGTTGTGCATCTACTTGGTTCGGTTGGTCTTGCTAAGCAGAAGACGGC12.5No Hit
GAGTTTTTTTTTTTTTCTTTCTTTCCCCCCCTTCTCTCCCCCCCCTTTTT12.5No Hit
TGTGTCCTCTGCTTGGTTCAAGTTGTTGGTCTTGCTAGCAGAAAGACATC12.5No Hit
TTGTGCTGGTCTACTCGTTCAGTTACGTATTGCTAATGATACGGCGACCA12.5No Hit
GTTATGTCCTCTACTTGGTTCAGTTACGTATTGCTAAGCAGAAGACGGCA12.5No Hit
ATATTTACTTCGTTCAGTTACGTATTGCTAAGCGAAGCAGGCATCGAGAT12.5No Hit
ATGTTATCTTGCTTCGTTCAGTTACGTATTGCTAATGATACGGCGACCAC12.5No Hit
TTGTAACCTACTCGTTCAGTTACGTATTGCTAATGATACGGCGACCACCG12.5No Hit
TGTGCACCTCGTTCAGTTACGTATTGCTTAGCGGAAGAAGCAGCATACAA12.5No Hit
ATGCCCTGTACTTGGTTCCATTACGTATTGCTAATGATACGGCGACCACC12.5No Hit
TTTTTTGTACTTCGTTCAGTTACGTATTGCTAATGATACTAGGCAACCAC12.5No Hit
TGTTTTTTTTTTTTTTTTTCCATCTCTGGCTAGGGTCATTGCTAATTGAT12.5No Hit
TTGTGCATTATATGTTGCTTACCTGGTTCAGTTACGTATTGCTAAGCAAA12.5No Hit
ATGTTCATTTTACTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCAT12.5No Hit
ATTCCCTCCTTACTTGGTTCAGTTAGTGTATTGCTAATGATACAGGCGAC12.5No Hit
TGTTATGCCCTGTTCTTGGTTCAGTTACGTATTGCTAAGCAGAAAAACAG12.5No Hit
ATGTCCTACTCGTTCAGTTACGTATTGCTAATGATACAGCAACCACCAGA12.5No Hit
TTTTTGTTGCTTCGTTCAGTTACGTATTGCTAAGCAGAAGACGGCATACG12.5No Hit
GTCCTCTACTTCGTTCAGTTACGTATTGCTAAGCGGAAGAGGACGACAGT12.5No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT12.5No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)