FastQCFastQC Report
Thu 14 Mar 2024
AA02_3276_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3276_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163418
Sequences flagged as poor quality0
Sequence length72-131820
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATCCATTCCCT10060.6155992607913449No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATCCATTCCCTCCGAT7800.4773036018064105No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATCCATTCCCT3530.21601047620213196No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATCCATTCC2620.16032505599138405No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATCCATTCCCTCCGATA2560.15665348982364244No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATCCATTCCCTC2290.14013144206880515No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATCCATTCCCTCCG1910.11687818967310822No Hit
GTTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATCCATTC1690.10341578039138896No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAATCCATTCCCT1680.10280385269676535No Hit
TTTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATCCATTCCCT1660.10157999730751814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)