FastQCFastQC Report
Thu 14 Mar 2024
AA02_3274_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3274_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208096
Sequences flagged as poor quality0
Sequence length68-101787
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGACAA12760.6131785329847763No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGACAAAGGTT9630.46276718437644165No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGACAA4130.1984660925726588No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGAC3450.16578886667691833No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGACAAAGGTTT2950.14176149469475627No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGACAAA2680.12878671382438875No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGACAAAG2490.11965631247116716No Hit
ATGTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGACA2340.11244810087651853No Hit
GTTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGA2330.11196755343687528No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGACAAAGG2320.11148700599723205No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGACAA2300.11052591111794556No Hit
TTTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGACAA2180.10475934184222666No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGAGGACAA2110.10139550976472397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)