FastQCFastQC Report
Thu 14 Mar 2024
AA02_3257_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3257_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230634
Sequences flagged as poor quality0
Sequence length69-137970
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACAGACGACT14510.6291353399758925No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACAGACGACTACAAA14130.6126590181846563No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACAGACGACT5170.2241646938439259No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACAGACGACTACAAAC3930.17039985431462837No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACAGACGACTACA3740.1621616934190102No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACAGACGA3680.1595601689256571No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACAGACGACT2890.1253067630965079No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACAGACGACTAC2670.11576783995421316No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACAGACGACTA2470.10709609164303614No Hit
ATGTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACAGACGAC2390.10362739231856534No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACAGACGACT2380.1031938049030065No Hit
ATGTACCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACAGACGAC2330.10102586782521224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)