FastQCFastQC Report
Thu 14 Mar 2024
AA02_3020_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3020_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156158
Sequences flagged as poor quality0
Sequence length87-188592
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATTGCGTCCTG9200.5891468896886487No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATTGCGTCCTGTTACG7180.4597907247787497No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATTGCGTCCTG2810.1799459521766416No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATTGCGTCC2350.15048860769220918No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATTGCGTCCTGT2120.13575993544999296No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATTGCGTCCTGTTACGA2040.13063691901791774No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATTGCGTCCTGTTA2040.13063691901791774No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAATTGCGTCCTG2000.12807541080188015No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATTGCGTCCTGTT1770.11334673855966393No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATTGCGTCCTG1660.10630259096556052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)