FastQCFastQC Report
Thu 14 Mar 2024
AA02_3017_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3017_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180286
Sequences flagged as poor quality0
Sequence length73-91617
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCGTG12500.693342799773693No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCGTGCAGTG9260.5136283460723516No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCGTG3990.2213150216877628No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCG2840.15752748410858303No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCGTGCAGTGT2820.15641813562894513No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCGTGCAG2460.13644986299546277No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCGTG2350.13034844635745427No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCGTGC2320.1286844236379974No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCGTGCA2290.12702040091854055No Hit
ATGTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCGT2100.1164815903619804No Hit
TTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCGTGC2030.11259887068324774No Hit
TTTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCGTG1950.1081614767646961No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGTTCCTCGTG1890.10483343132578236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)