FastQCFastQC Report
Thu 14 Mar 2024
AA02_3007_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3007_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115262
Sequences flagged as poor quality0
Sequence length70-85809
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACCCTCCAGGAAAGT6220.5396401242386909No Hit
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACCCTCCAGG6070.5266262948760216No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACCCTCCAGG2030.17612049070812583No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACCCTCCAGGAAAGTA1730.15009283198278706No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACCCTCCA1680.14575488886189725No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACCCTCCAGGAAA1470.1275355277541601No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACCCTCCAGG1220.10584581214971109No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACCCTCCAGGAA1220.10584581214971109No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACCCTCCAGG1170.10150786902882128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)