FastQCFastQC Report
Thu 14 Mar 2024
AA02_2974_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_2974_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69143
Sequences flagged as poor quality0
Sequence length74-86707
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACGAGTCTC3880.5611558653804434No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACGAGTCTCTTGGG2720.39338761696773356No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACGAGTCTC1250.1807847504447305No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACGAGTC1050.15185919037357362No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACGAGTCTC910.13161129832376378No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACGAGTCTCTTGGGA870.12582618630953243No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACGAGTCTCTTG840.12148735229885889No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACGAGTCTCT800.11570224028462751No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACGAGTCTC770.11136340627395397No Hit
GTTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACGAGT700.10123946024904906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)