FastQCFastQC Report
Fri 15 Mar 2024
AA02_3273_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3273_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197876
Sequences flagged as poor quality0
Sequence length73-91914
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACCAAGACT12120.6125048009864764No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACCAAGACTCGCTG9430.4765610786553195No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACCAAGACT4410.22286684590349512No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACCAAGACTCGCTGT3390.1713194121571085No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACCAAGA3000.1516100992540783No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACCAAGACTCGC2600.13139541935353452No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACCAAGACTC2570.12987931836099376No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACCAAGACTCG2370.11977197841072187No Hit
ATGTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACCAAGAC2150.1086539044654228No Hit
TTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACCAAGACTC2140.10814853746790919No Hit
TTTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACCAAGACT2040.10309486749277325No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAACCAAGACT2000.10107339950271886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)