FastQCFastQC Report
Fri 15 Mar 2024
AA02_3268_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3268_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190067
Sequences flagged as poor quality0
Sequence length74-105036
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACGTAACTTG10410.5477015999621186No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACGTAACTTGGTTTG7270.38249669853262275No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACGTAACTTG4100.21571340632513797No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACGTAACT2750.1446858213156413No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACGTAACTTGG2350.12364061094245714No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACGTAACTTGGTT2200.11574865705251307No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACGTAACTTGGTTTGT2140.11259187549653545No Hit
GTTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACGTAAC2030.10680444264390977No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAACGTAACTTGGT1920.10101700979128414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)