FastQCFastQC Report
Fri 15 Mar 2024
AA02_3265_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3265_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230482
Sequences flagged as poor quality0
Sequence length70-102302
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGATTCAT13840.600480731684036No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGATTCATTCCCA11050.47943006395293347No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGATTCAT5120.2221431608542099No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGATTCATTCCCAC3620.15706215669770307No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGATTCATT3330.1444798292274451No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGATTC3300.14317820914431498No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGATTCATTCC2910.12625714806362318No Hit
TTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGATTCATT2750.11931517428692914No Hit
TTTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGATTCAT2680.11627806075962548No Hit
GTTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGATT2670.11584418739858211No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGATTCATTC2520.10933608698293143No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGATTCAT2370.10282798656728075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)