FastQCFastQC Report
Fri 15 Mar 2024
AA02_3263_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3263_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134497
Sequences flagged as poor quality0
Sequence length67-62553
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGTTACACAAACC8780.6528026647434515No Hit
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGTTACAC8320.61860115839015No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGTTACAC2850.2119006371889336No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGTTAC2350.17472508680491014No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGTTACACAAACCC2210.16431593269738357No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGTTACACAAA2030.15093273455913514No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGTTACACA1770.13160144835944296No Hit
ATGTACCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGTTACA1570.11673122820583359No Hit
GTTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGTTA1530.11375718417511171No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGTTACAC1520.11301367316743124No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGTTACAC1510.11227016215975076No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAAGGTTACACAA1470.1092961181290289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)