FastQCFastQC Report
Fri 15 Mar 2024
AA02_3259_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3259_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119074
Sequences flagged as poor quality0
Sequence length65-71136
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATAGGGAAACA7570.6357391202109612No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATAGGGAAACACGATA6220.5223642440835111No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATAGGGAAACA2440.20491459092665068No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATAGGGAAA2060.17300166283151652No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATAGGGAAACACGATAG2030.17048222113979541No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATAGGGAAACAC1970.1654433377563532No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATAGGGAAACACGA1950.16376370996187245No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAATAGGGAAACA1520.12765171238053646No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATAGGGAAACACG1460.12261282899709423No Hit
GTTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATAGGGAA1400.11757394561365202No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAATAGGGAAACA1360.11421469002469053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)