FastQCFastQC Report
Fri 15 Mar 2024
AA02_3258_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3258_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154004
Sequences flagged as poor quality0
Sequence length83-90696
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCTGGTAACT10920.7090724916235942No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCTGGTAACTGGGAC8850.5746603984312095No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCTGGTAACT4110.266876185034155No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCTGGTAA2750.17856679047297472No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCTGGTAACTGGG2580.1675281161528272No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCTGGTAACTG2390.1551907742656035No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCTGGTAACTGGGACA2340.15194410534791306No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCTGGTAACT1950.12662008778992753No Hit
GTTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCTGGTA1730.11233474455208955No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCTGGTAACTGG1710.11103607698501339No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCTGGTAACT1560.10129607023194204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)