FastQCFastQC Report
Fri 15 Mar 2024
AA02_3256_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3256_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178043
Sequences flagged as poor quality0
Sequence length73-102210
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGACA11030.6195132636497924No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGACACCGAC8490.47685109776851653No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGACA3870.21736322124430613No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGACACCGACA2810.15782704178204143No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGA2730.15333374521885162No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGACAC2640.1482787865852631No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGACACC2360.13255224861409884No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGACACCG2260.1269356279101116No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGACA1930.10840077958695371No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGACA1860.10446914509416264No Hit
ATGTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGAC1860.10446914509416264No Hit
TTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGACAC1840.10334582095336521No Hit
TTTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACACAAAGACA1840.10334582095336521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)