FastQCFastQC Report
Fri 15 Mar 2024
AA02_3098_2a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3098_2a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168679
Sequences flagged as poor quality0
Sequence length72-124815
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGCATAGTTCT10130.600548971715507No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGCATAGTTCTGCATG8770.5199224562630795No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGCATAGTTCT2970.17607408153949217No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGCATAGTT2770.16421724103178226No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGCATAGTTCTGCA2580.15295324254945786No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGCATAGTTCTGCATGA2540.15058187444791585No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGCATAGTTCTG2190.12983240355942352No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGCATAGTTCT2120.12568250938172504No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGCATAGTTCT2080.12331114128018307No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGCATAGTTCTGC1820.10789724862016019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)