FastQCFastQC Report
Fri 15 Mar 2024
AA02_3027_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3027_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25090
Sequences flagged as poor quality0
Sequence length89-82171
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGCCTCTCA1470.5858907931446791No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGCCTCTCATTGTC1470.5858907931446791No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGCCTCTCATTGTCC500.19928258270227184No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGCCTCTCATTG440.1753686727779992No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGCCTCTCA440.1753686727779992No Hit
GTTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGCCTC340.13551215623754484No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGCCTCT320.12754085292945397No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGCCTCTCAT320.12754085292945397No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGCCTCTCA310.12355520127540853No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAAGAGCCTCTCA290.11558389796731765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)