FastQCFastQC Report
Fri 15 Mar 2024
AA02_3012_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3012_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49159
Sequences flagged as poor quality0
Sequence length80-112970
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCAA3060.6224699444659167No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCAACAACC2600.5288960312455502No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCAA1170.23800321406049757No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCAACAACCT900.18307939543115198No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCAACAA780.15866880937366507No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCC750.1525661628592933No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCAA730.14849773184971216No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCAA700.14239508533534043No Hit
ATGTCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCA610.12408714579222524No Hit
GTTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACC560.11391606826827234No Hit
TTTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCAACAACCT560.11391606826827234No Hit
ATGTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCA540.10984763725869118No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCAAC530.1078134217539006No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCAACA520.10577920624911002No Hit
ATGTACCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACCAAACCCA500.10171077523952889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)