FastQCFastQC Report
Fri 15 Mar 2024
AA02_3003_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_3003_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53764
Sequences flagged as poor quality0
Sequence length67-91633
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAACTGA3710.6900528234506361No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAACTGACAGTG3000.5579941968603526No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAACTGA1160.21575775611933634No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAACT980.18227810430771518No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAACTGAC840.15623837512089875No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAACTGA760.14135852987128933No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAACTGACAG710.13205862659028345No Hit
GTTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAAC670.12461870396547876No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAACTGACA650.12089874265307642No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAACTGACAGTGG650.12089874265307642No Hit
ATGTCCTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAACTGA590.10973885871586936No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAACTGA560.10415891674726582No Hit
TTTACTTCGTTCAGTTACGTATTGCTAAGGTTAACGTCAACTGACAGTGG550.10229893609106466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)