FastQCFastQC Report
Fri 15 Mar 2024
AA02_2994_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_2994_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154803
Sequences flagged as poor quality0
Sequence length76-86712
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTACCT10390.6711756232114364No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTACCTCGCTA8410.543271125236591No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTACCT3840.24805720819363963No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTAC2740.17699915376316996No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTACCTCGCTAA2560.1653714721290931No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTACCTCGC2350.15180584355600343No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTACCTC2330.1505138789299949No Hit
GTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTACCTCG1860.12015271021879421No Hit
GTTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTA1810.11692279865377286No Hit
TTTTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTACCT1810.11692279865377286No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTACCT1680.10852502858471735No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTACCT1600.1033571700806832No Hit
ATGTACCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGGTCTACC1560.10077324082866611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)