FastQCFastQC Report
Fri 15 Mar 2024
AA02_2968_a_merged.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAA02_2968_a_merged.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82603
Sequences flagged as poor quality0
Sequence length82-162166
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGAACGACTT5170.6258852584046584No Hit
ATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGAACGACTTCCATA4200.5084561093422757No Hit
ATGCCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGAACGACTT1840.2227522002832827No Hit
TTATGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGAACGAC1370.16585354042831374No Hit
TGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGAACGACTTCCATAC1240.15011561323438616No Hit
TTATGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGAACGACTTCCA1190.14406256431364478No Hit
TGTCCTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGAACGACTTC1080.13074585668801375No Hit
ATGTACTGTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGAACGACTT900.10895488057334479No Hit
ATGTAGTCTACTTCGTTCAGTTACGTATTGCTAAGGTTAAAGAACGACTT880.10653366100504824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)