FastQCFastQC Report
Mon 14 Oct 2024
000000000-LRD8L_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LRD8L_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16695201
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC351020521.02523353866779Illumina Single End PCR Primer 1 (100% over 8bp)
GTCAGATA9200775.5110267914713935No Hit
TCGACGAG9181215.499310849866378No Hit
CTACTATA8534655.112037884419601No Hit
CGTTACTA8396145.029073923698194No Hit
ACGTCTCG8324024.985875881338595No Hit
GATCGTGT7929504.74956845383293No Hit
TACGAGAC7809344.677595675547722TruSeq Adapter, Index 14 (100% over 8bp)
AGAGTCAC7340454.396742513013171No Hit
GACACCGT4453092.667287443858867No Hit
CTGCGTGT4372662.6191119232406965No Hit
TCATCGAG4302132.576866250367396No Hit
TAGCGAGT4188422.5087568577341477No Hit
CGTGAGTG4024772.4107346775878886No Hit
ACTATCTG3666282.1960083020264327No Hit
GGATATCT3491952.0915890740099505No Hit
ATCGTACG3405082.0395561574850163RNA PCR Primer, Index 22 (100% over 8bp)
TAGTGTAG2714051.625646795147899No Hit
GACACTGA2528801.5146867653764695No Hit
GCTCTAGT2445091.4645466083337362No Hit
CGTCGCTA2398001.4363408982018246No Hit
TGCGTACG2198901.317085071332774No Hit
CTAGAGCT2082571.2474063654579541No Hit
ATATACAC1712191.025558182857457No Hit
ACGACGTG1373280.8225597283914101No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)