FastQCFastQC Report
Mon 14 Oct 2024
000000000-LRD8L_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LRD8L_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16695201
Sequences flagged as poor quality0
Sequence length8
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGTACC6997904.191563791295475No Hit
CGAAGTAT5887503.5264624846385497No Hit
GATCTACG5779803.4619529288686017No Hit
CTCGACTT5556973.328483436647453No Hit
TAGCAGCT5416733.244483250006993No Hit
TCTCTATG4795752.872532052773728ABI Solid3 Adapter B (100% over 8bp)
GCGTATAC4575112.740374314750688No Hit
ACGTGCGC4356402.6093725975506374No Hit
TGCTCGTA3945742.3633977212972757No Hit
GTAACGAG3754682.248957649566483No Hit
ATGAGCTC3155541.8900880558431132No Hit
CGAGCTAG3117691.8674168702730802No Hit
CTCTAGAG2963261.7749172351983065No Hit
ATAGCGCT2961681.7739708554572058No Hit
AGCATACC2936091.7586430974984968No Hit
TCTAGACT2919991.748999607731587No Hit
TCCTCATG2894541.7337557062056335No Hit
TCAGTCTA2808911.6824655180851071No Hit
CGTCATAC2797611.6756971060126797No Hit
TAGTCTCC2699001.6166322286266572No Hit
ACTCACTG2515381.5066485273223125No Hit
CGAGAGTT2327251.3939634509341936TruSeq Adapter, Index 13 (100% over 8bp)
ACGCTACT2319261.3891776445219197No Hit
GACATAGT2269911.3596182519755229No Hit
ACTACGAC2242581.3432482783525637No Hit
CGAGCGAC2138991.2812005078585158No Hit
TGAGTACG2102571.2593858558516307No Hit
CATCGTGA2096191.2555643984160478No Hit
CTGCGTAG2032861.2176313420844709No Hit
AACGCTGA1958391.173025709603616No Hit
GTCTGCTA1939211.1615373783160803No Hit
CAGTAGGT1687971.0110510199907148No Hit
CGTAGCGA1359610.8143717467073323No Hit
AGTCGCAG1274010.7630995278223963No Hit
CTATCGTG1109950.6648317681230672No Hit
TACTAGGT1104170.6613696953992947No Hit
ACGTACGT1065360.6381234942903652No Hit
GTTACAGC1058120.6337869187678543No Hit
CTACGACC1033560.6190761045644194No Hit
TAACGTCC1000590.5993279146504435No Hit
CGCGATAT992200.5943025184302962No Hit
GCGATACG915990.5486546702851915No Hit
GAGACTTA826890.49528604058136233No Hit
ACGTATAC612450.36684194458036173No Hit
AATCTACG428640.25674443811727693No Hit
ATAACGAG331570.19860198149156758No Hit
GTCTATGA318000.19047389725945799No Hit
TATAGCGA313670.18788033758922698No Hit
ACTGTGTA279980.16770088602107877No Hit
AAAAAAAA245330.14694641891403404No Hit
TTTTTTTT179830.10771358787474317No Hit
GAGCTCGA167760.10048396542215933No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)