FastQCFastQC Report
Wed 19 Feb 2025
000000000-LPC4P_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LPC4P_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7460464
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGTGT86985711.659556295694209No Hit
CGTGAGTG79813610.698208583273104No Hit
TCATCGAG75535010.12470538025517No Hit
GGATATCT6872969.21251010660999No Hit
GACACCGT6418508.603352284790866No Hit
TCTTTCCC5879127.880367762648543Illumina Single End PCR Primer 1 (100% over 8bp)
ACTATCTG5352697.174741410185748No Hit
ATCGTACG5077026.805233561880334RNA PCR Primer, Index 22 (100% over 8bp)
TAGCGAGT3646014.887108898320534No Hit
CGTGAGTT231320.31006114365004644No Hit
ACTATCTT201010.26943364380553275No Hit
CCATCGAG170100.22800190443918766No Hit
TCATCGAT164590.22061630483036979No Hit
CGTTACTA154380.20693082896720635No Hit
TCGACGAG150300.20146200021875313No Hit
GTCAGATA146300.19610040340654417No Hit
TACACCGT146290.1960869994145136No Hit
CCTATCTG135900.18216025169480074No Hit
ACGTCTCG119960.16079428839814788No Hit
CTACTATA118810.1592528293146378No Hit
CTCGTACG109160.1463179770051836No Hit
TGATATCT108650.14563437341162694TruSeq Adapter, Index 25 (100% over 8bp)
TTGCGTGT105130.14091616821688302No Hit
CGTGCGTG102570.13748474625706927No Hit
CACACCGT96450.1292815031343895No Hit
AGAGTCAC91410.12252589115100615No Hit
GATCGTGT87290.11700344643443088No Hit
ATCGTACT86700.11621261090463005No Hit
TAGCGTGT86420.11583729912777543No Hit
GGCTATCT85400.11447009194066214No Hit
GTATATCT81180.10881360730378165No Hit
GACACCGG78770.10558324522442572No Hit
CTGCGTTT78370.10504708554320483No Hit
TACGAGAC78100.10468517775838071TruSeq Adapter, Index 14 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)