FastQCFastQC Report
Wed 19 Feb 2025
000000000-LPC4P_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LPC4P_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7460464
Sequences flagged as poor quality0
Sequence length8
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCT98428213.193308083786745No Hit
CGAAGTAT91683112.289195417335973No Hit
TCTCTATG79216910.618226962826977ABI Solid3 Adapter B (100% over 8bp)
GATCTACG7428889.957664831570797No Hit
GTAACGAG6601768.848993842742221No Hit
CTCGACTT3525844.726033126089744No Hit
CGTCATAC1033041.3846859927210962No Hit
CGAGCTAG1004791.34681971523487No Hit
ATAGCGCT981741.3159235136045158No Hit
TCTAGACT962921.2906972006030724No Hit
TCCTCATG921501.2351778656126482No Hit
CAGTAGGT877431.1761064727341355No Hit
CATCGTGA812051.0884711728385794No Hit
AGCATACC807041.0817557728312877No Hit
CTCTAGAG796621.0677888131354834No Hit
TCAGTCTA634200.8500811745757367No Hit
GAGCTCGA630680.8453629693809929No Hit
ATGAGCTC467770.626998535211751No Hit
TATCTACG268750.36023228582029215No Hit
TCTCTATT210610.28230147615483436No Hit
TAGCAGCG196820.2638173711447438No Hit
TCTCTACG171920.2304414309887428RNA PCR Primer, Index 17 (100% over 8bp)
GCTCTACG159850.21426281260790214No Hit
TAGCATCT151450.20300345930226324RNA PCR Primer, Index 24 (100% over 8bp)
TATCAGCT127100.17036473870794094No Hit
CTAAGTAT126350.16935943930565178No Hit
CGAAGTAG125350.16801904010259952No Hit
GTAACGCG121020.16221511155338328No Hit
TGAAGTAT114700.15374378859009305No Hit
TTAACGAG103890.13925407320509825No Hit
GCTCTATG103290.13844983368326688No Hit
CGACGTAT102620.13755176621722187No Hit
TATCTACT89100.11942956899195546No Hit
GTAACGAT88830.11906766120713135No Hit
TCTCTCTG88810.11904085322307031No Hit
TCTCTTTT88700.11889340931073455No Hit
TAGCCGCT87760.11763343405986545No Hit
GATCTACT85250.11426903206020429No Hit
GTTCTACG83490.11190992946283233No Hit
CGTAGTAT80720.10819702367037759No Hit
CGCAGTAT80180.10747320810072938No Hit
TCGCAGCT79450.10649471668250125No Hit
TCTCTCTT79160.10610600091361609No Hit
CGCGATAT78770.10558324522442572No Hit
CTAACTAT77510.10389434222857988No Hit
CGAAGTCT77050.10327775859517586No Hit
CGAATTAT77040.10326435460314533No Hit
ACGTACGT76710.10282202286613809No Hit
TTTCTTTT76620.10270138693786338No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)