FastQCFastQC Report
Wed 5 Mar 2025
000000000-LNWH6_l01_n02_toy_overexpression.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LNWH6_l01_n02_toy_overexpression.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8275392
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT1045361.2632150839501017Illumina Single End PCR Primer 1 (96% over 32bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGCAGAAATC325710.3935886058328113Clontech Universal Primer Mix Long (100% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT291650.3524304347153585Illumina Single End PCR Primer 1 (96% over 32bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGATAATTTT276180.3337364562307139Clontech Universal Primer Mix Long (100% over 25bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT191270.23113104491001754Clontech SMART CDS Primer II A (100% over 26bp)
CGTACGTACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA148040.1788918277224813Illumina Single End PCR Primer 1 (96% over 33bp)
TACGTACGTACGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCC132250.1598111606072558Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC105940.1280181047616838Illumina Single End PCR Primer 1 (96% over 33bp)
TACGTACGTACGTACGTACGTAAGATCGGAAGAGCGTCGTGTAGGGAAAG102430.12377661384499973Illumina Single End PCR Primer 1 (100% over 28bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102220.12352284943118104Clontech SMART CDS Primer II A (100% over 26bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGATTCGCA96220.11627243760778945Clontech Universal Primer Mix Long (100% over 25bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGAGATCGGA83940.10143326140924804Clontech Universal Primer Mix Long (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)