FastQCFastQC Report
Wed 5 Mar 2025
000000000-LNWH6_l01_n02_Control.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LNWH6_l01_n02_Control.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11513309
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT1332841.1576515491766963Illumina Single End PCR Primer 1 (97% over 34bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT289150.25114413241232386Clontech SMART CDS Primer II A (100% over 26bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGCAGAAATC285380.24786966110264216Clontech Universal Primer Mix Long (100% over 25bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGATAATTTT219120.190318873574921Clontech Universal Primer Mix Long (100% over 25bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGAGATCGGA141820.12317918332600993Clontech Universal Primer Mix Long (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCAGATCGGA125530.10903034045208028Clontech SMART CDS Primer II A (96% over 26bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGATTCGCA124020.10771881480814943Clontech Universal Primer Mix Long (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)