FastQCFastQC Report
Wed 5 Mar 2025
000000000-LNWH6_l01_n01_toy_overexpression.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LNWH6_l01_n01_toy_overexpression.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8275392
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT1767422.135753810816454Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT1610491.9461192895756478TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1034491.2500797545300573Clontech SMART CDS Primer II A (100% over 26bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGCAGAAATC316170.3820604510336187Clontech Universal Primer Mix Long (100% over 25bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGATAATTTT267170.32284875447592093Clontech Universal Primer Mix Long (100% over 25bp)
CGTACGTACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA244900.2959376425914325TruSeq Adapter, Index 6 (97% over 36bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT171720.20750678638546668Clontech SMART CDS Primer II A (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA156670.18932033672845952TruSeq Adapter, Index 6 (97% over 36bp)
TACGTACGTACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC156200.18875238780229384TruSeq Adapter, Index 5 (97% over 35bp)
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT103240.12475541944115759Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94870.11464109494752635No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGATTCGCA93270.11270765179462194Clontech Universal Primer Mix Long (100% over 25bp)
TACGTACGTACGTACGTACGTAAGATCGGAAGAGCACACGTCTGAACTCC91990.1111608972722984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCAGATCGGA91370.11041168805054793Clontech SMART CDS Primer II A (96% over 26bp)
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT91100.1100854195184953Clontech SMART CDS Primer II A (100% over 26bp)
TACGTACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA86830.10492554310418165TruSeq Adapter, Index 6 (97% over 36bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86070.10400715760655205No Hit
TACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTA83610.10103448875896151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)