FastQCFastQC Report
Wed 5 Mar 2025
000000000-LNWH6_l01_n01_Control.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LNWH6_l01_n01_Control.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11513309
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT2012981.7483939673642042Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1255971.0908853397402953TruSeq Adapter, Index 27 (97% over 39bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT484250.42060019408842414Clontech SMART CDS Primer II A (100% over 26bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGCAGAAATC271400.235727191895918Clontech Universal Primer Mix Long (100% over 25bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGATAATTTT203530.17677802272135665Clontech Universal Primer Mix Long (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCAGATCGGA145100.1260280602214359Clontech SMART CDS Primer II A (96% over 26bp)
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT136490.11854975837094271Clontech SMART CDS Primer II A (100% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130440.11329497019492833No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGAGATCGGA126240.10964701807273651Clontech Universal Primer Mix Long (100% over 25bp)
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT119720.1039840066830483Clontech Universal Primer Mix Long (96% over 26bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGATTCGCA115330.10017102815532877Clontech Universal Primer Mix Long (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)