FastQCFastQC Report
Mon 23 Sep 2024
000000000-LNMRD_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LNMRD_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5873732
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC296628350.50082298613556Illumina Single End PCR Primer 1 (100% over 8bp)
TCGACGAG1767193.0086323311993124No Hit
GTCAGATA1759762.995982792541437No Hit
CGTTACTA1627272.7704192155855933No Hit
GATCGTGT1562312.659825133322392No Hit
CTACTATA1543492.627784175376064No Hit
TACGAGAC1299802.212903142329272TruSeq Adapter, Index 14 (100% over 8bp)
ACGTCTCG1221472.0795467004623296No Hit
CTGCGTGT1099281.8715188231264213No Hit
GACACCGT1085141.847445542288957No Hit
AGAGTCAC1084031.8455557727182648No Hit
TCATCGAG1024011.743372016292197No Hit
TAGCGAGT954831.6255934046701483No Hit
CGTGAGTG943221.6058274364577751No Hit
GGATATCT766911.305660523837315No Hit
ACTATCTG750711.2780801030758637No Hit
ATCGTACG690391.1753855981171766RNA PCR Primer, Index 22 (100% over 8bp)
TAGTGTAG635631.0821569659630368No Hit
GACACTGA532470.9065275705462897No Hit
GCTCTAGT523580.8913923890296663No Hit
CGTCGCTA510850.8697196262955136No Hit
TGCGTACG501140.8531883987897303No Hit
CTAGAGCT431430.7345074647600538No Hit
CCGTCTCG335150.5705912356913798TruSeq Adapter, Index 14 (100% over 8bp)
ATATACAC260630.4437213001887046No Hit
ACGACGTG233930.3982646807855721No Hit
CGAGTCAC229090.3900246044593114No Hit
CCTATCTG183240.3119652037239697No Hit
TCTTTCAC170040.2894922682887132No Hit
CTCGTACG153230.2608733255109358No Hit
TACGCGAC132710.22593812587976433No Hit
CGTTACTC93880.15983024080771815No Hit
TATTTCCC89640.15261166154669636No Hit
CGTGCGTG88020.14985361947055126No Hit
TCTTTACC87870.14959824520424153No Hit
TCGACGCG85780.1460400304269926No Hit
CGTTCCTA80660.13732325547028704No Hit
GCTCGTGT78690.13396934010608585No Hit
CTCCTATA64260.10940233568708957No Hit
CTACTCTA61230.10424377550763296No Hit
GTCAGCTA60690.10332442814891792No Hit
GTCAGATC60600.1031712035891321No Hit
CCGACGTG59760.10174110769779758No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)