FastQCFastQC Report
Mon 23 Sep 2024
000000000-LNMRD_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LNMRD_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5873732
Sequences flagged as poor quality0
Sequence length8
%GC28

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAA65853311.211492114383155No Hit
ATAGTACC1350112.2985556712495563No Hit
CGAAGTAT1082971.8437511279030094No Hit
TAGCAGCT1081211.840754736511642No Hit
CTCGACTT999931.7023759340739415No Hit
GCGTATAC927611.5792514878104755No Hit
GATCTACG902971.53730200833133No Hit
ACGTGCGC815121.3877378130292632No Hit
ATGAGCTC771671.3137644005548772No Hit
TCTCTATG767811.3071927694351735ABI Solid3 Adapter B (100% over 8bp)
AAAAATAA734231.2500229836839678No Hit
TGCTCGTA719971.2257454034334558No Hit
GTAACGAG701311.1939768447045251No Hit
AATAAAAA691831.17783719107375No Hit
CGTCATAC691441.1771732179813448No Hit
CTCTAGAG690601.1757431220900103No Hit
ATAGCGCT688641.1724062316768964No Hit
TCTAGACT676261.1513293422307997No Hit
AGCATACC662001.1270517619802878No Hit
CGAGCTAG652981.1116952560995292No Hit
TCAGTCTA640141.0898352189034162No Hit
TCCTCATG614851.0467791176035952No Hit
AAAATAAA574660.978355839183674No Hit
CAAAAAAA568180.9673236708790935No Hit
AAAAAATA542170.9230417731009858No Hit
AAAAACAA497040.8462081688439309No Hit
CATCGTGA465120.7918645249732197No Hit
TAAAAAAA465050.7917453503156086No Hit
AAAACAAA454630.7740053512826257No Hit
AAATAAAA435880.7420835679939092No Hit
CAGTAGGT424630.7229304980206792No Hit
AAAAAAAT401670.6838412103242028No Hit
AACGCTGA398740.6788528996556192No Hit
ACAAAAAA398160.6778654524925549No Hit
AACAAAAA397790.6772355293023243No Hit
TACTAGGT366210.6234707337685819No Hit
AAGAAAAA365840.6228408105783512No Hit
AAAAAACA365150.6216660889533264No Hit
AGTCGCAG353240.6013893722083336No Hit
AAACAAAA352050.5993634030289431No Hit
ACGTACGT335360.5709487596642135No Hit
GTTACAGC334110.5688206407782991No Hit
CTACGACC333740.5681907175880684No Hit
AAAAAGAA330400.562504383924905No Hit
TAGTCTCC325750.5545877816693033No Hit
TAACGTCC309980.5277394338046068No Hit
CGCGATAT303670.5169966896685106No Hit
CTATCGTG300890.5122637532662369No Hit
ACTACGAC289660.49314473319518154No Hit
AAAAAAAC286570.48788402330920105No Hit
GACATAGT283340.48238496410799814No Hit
ACTCACTG276140.47012699932513097No Hit
GAGACTTA272880.4645768652706661No Hit
CGAGAGTT271820.4627722204554106TruSeq Adapter, Index 13 (100% over 8bp)
CGTAGCGA266960.45449809422697535No Hit
ACGCTACT265540.4520805511725765No Hit
CGAGCGAC262980.4477221636942237No Hit
AAAAGAAA262440.4468028163355087No Hit
GCGATACG245810.41849032267730296No Hit
AAAAAAAG236210.40214636963348No Hit
TGAGTACG214940.36593429867075994No Hit
GTCTGCTA214340.364912801605521No Hit
CTGCGTAG201230.34259309073005034No Hit
ATAAAAAA183890.3130718255446452No Hit
AAAGAAAA174400.29691514696278276No Hit
AATAATAA148150.2522246503585795No Hit
GAAAAAAA140690.23952403684744214No Hit
AATAAATA117640.20028152459117984No Hit
AATATAAA114730.195327263824771No Hit
ACGTATAC112090.19083267673771973No Hit
AAAATATA110320.1878192603952649No Hit
AAAAAAGA98820.16824056664485204No Hit
AAATATAA98540.16776386801440718No Hit
CAAAATAA98520.16772981811223256No Hit
TATAAAAA95760.16303093161213347No Hit
TAAAATAA88420.1505346175140439No Hit
ACTGTGTA79380.13514406173111065No Hit
CATAAAAA79110.13468438805175312No Hit
GATATACG78510.13366289098651418No Hit
TAAAAATA77650.13219874519300506No Hit
AGAAAAAA77590.13209659548648117No Hit
AATAACAA76180.12969607738316968No Hit
ATAATACC74540.12690398540484993No Hit
AAAAATTA73070.12440131759501455No Hit
AAAATTAA72380.12322659596998979No Hit
AAGAATAA72000.12257964782867178No Hit
AAAAATAT70770.12048557884493198No Hit
TAAATAAA68350.1163655406818016No Hit
AAATAATA68250.11619529117092846No Hit
AATCTACG67320.11461197071980812No Hit
AAAATACA65000.11066218206755092No Hit
AATAAAAT64400.10964068500231199No Hit
AAAACTAA63640.10834678871967601No Hit
AACAATAA63360.10787009008923117No Hit
CAAAACAA59810.10182623245323416No Hit
AACATACC59550.10138358372496396No Hit
AATACAAA59340.10102605975213033No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)