FastQCFastQC Report
Mon 28 Oct 2024
000000000-LMV92_l01_n02_HRG_01LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LMV92_l01_n02_HRG_01LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2557
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGATCT48418.92843175596402Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAG230.899491591709034Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGT210.8212749315604223Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGT180.7039499413375049Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGT180.7039499413375049Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGATCTC120.4692999608916699Illumina Single End PCR Primer 1 (96% over 28bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGGAGATCT110.4301916308173641Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTC100.3910833007430583Illumina Single End PCR Primer 1 (96% over 33bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGATC90.35197497066875244Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAG80.3128666405944466Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGATTT70.27375831052014077Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGA70.27375831052014077Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCGAAGTGTAGATCT70.27375831052014077Illumina Single End PCR Primer 1 (96% over 33bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGATC60.23464998044583496Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGCAGATCT60.23464998044583496Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAA50.19554165037152915Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTA50.19554165037152915Illumina Single End PCR Primer 1 (96% over 33bp)
AGTTCAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTCGTGTAGGG40.1564333202972233Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGATAT40.1564333202972233Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCACGAAGTGTAGATCT40.1564333202972233Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCG40.1564333202972233Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGATCTC40.1564333202972233Illumina Single End PCR Primer 1 (96% over 25bp)
GAAGAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGT30.11732499022291748Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTC30.11732499022291748Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTG30.11732499022291748Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGATCC30.11732499022291748Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCGCGAAGTGTAGATCT30.11732499022291748Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)