FastQCFastQC Report
Mon 28 Oct 2024
000000000-LMV92_l01_n02_HRG_01EB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LMV92_l01_n02_HRG_01EB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGATGGTGTAGATCT723.440038222646918Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGATGG140.6688963210702341Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGATGGT70.33444816053511706Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGATGGTGTAG70.33444816053511706Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGATGGT40.19111323459149546Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGATGGT40.19111323459149546Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)