FastQCFastQC Report
Mon 28 Oct 2024
000000000-LMV92_l01_n01_HRG_01LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LMV92_l01_n01_HRG_01LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2557
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATG86033.63316386390301TruSeq Adapter, Index 18 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTAT56221.978881501759876TruSeq Adapter, Index 18 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGCCG491.9163081736409855TruSeq Adapter, Index 18 (97% over 34bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTA451.759874853343762TruSeq Adapter, Index 18 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGC411.6034415330465388TruSeq Adapter, Index 18 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGCCGTC391.5252248728979272TruSeq Adapter, Index 18 (96% over 32bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCT271.0559249120062575TruSeq Adapter, Index 18 (97% over 37bp)
AGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGCCGTCTTCT261.0168165819319515TruSeq Adapter, Index 18 (96% over 28bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTA240.9385999217833398TruSeq Adapter, Index 18 (97% over 37bp)
AGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGCCGTCTT230.899491591709034TruSeq Adapter, Index 18 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATATCGTATG180.7039499413375049TruSeq Adapter, Index 18 (97% over 37bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGCC140.5475166210402815TruSeq Adapter, Index 18 (97% over 35bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC120.4692999608916699TruSeq Adapter, Index 1 (100% over 36bp)
GATCGGAAGAGGACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATG110.4301916308173641TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATACTCTATCTCGTATG110.4301916308173641TruSeq Adapter, Index 27 (97% over 37bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTC100.3910833007430583TruSeq Adapter, Index 18 (97% over 37bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTA100.3910833007430583TruSeq Adapter, Index 18 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATATCGTAT90.35197497066875244TruSeq Adapter, Index 18 (97% over 37bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATG90.35197497066875244TruSeq Adapter, Index 1 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGCCGT80.3128666405944466TruSeq Adapter, Index 18 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCTCTATCTCGTAT80.3128666405944466TruSeq Adapter, Index 13 (97% over 37bp)
GACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCT70.27375831052014077TruSeq Adapter, Index 18 (97% over 37bp)
ACACTCTTTCCCCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTAT70.27375831052014077TruSeq Adapter, Index 18 (96% over 26bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTA60.23464998044583496TruSeq Adapter, Index 18 (97% over 37bp)
ACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGCCGTCTTCTGCT60.23464998044583496Illumina PCR Primer Index 2 (96% over 25bp)
GATCGGAAGAGACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGC60.23464998044583496TruSeq Adapter, Index 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCTCTATCTCGTATG60.23464998044583496TruSeq Adapter, Index 13 (97% over 37bp)
GGGGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGCCGTC50.19554165037152915TruSeq Adapter, Index 18 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTACCTCGTATG50.19554165037152915TruSeq Adapter, Index 18 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTAGATCGGAAGAGCACACGTCTGAA50.19554165037152915Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTC50.19554165037152915TruSeq Adapter, Index 18 (97% over 37bp)
CCAGTCACAATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTAT40.1564333202972233TruSeq Adapter, Index 1 (97% over 36bp)
AGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCT40.1564333202972233TruSeq Adapter, Index 18 (97% over 37bp)
GAAGAAGACTAGCTGAGCTCTCGGAAGAGCACACGTCTGAACTCCAGTCA40.1564333202972233Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTAT40.1564333202972233TruSeq Adapter, Index 1 (97% over 36bp)
GTGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC40.1564333202972233TruSeq Adapter, Index 1 (100% over 36bp)
GATCGGAAAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGC40.1564333202972233TruSeq Adapter, Index 18 (96% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGCAT40.1564333202972233TruSeq Adapter, Index 18 (97% over 37bp)
AGATCGGAAGAGCCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTA40.1564333202972233TruSeq Adapter, Index 1 (96% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATCTATCTCGTATG30.11732499022291748TruSeq Adapter, Index 1 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATATCGTATGC30.11732499022291748TruSeq Adapter, Index 18 (97% over 36bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATG30.11732499022291748TruSeq Adapter, Index 1 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATACTCTATCTCGTAT30.11732499022291748TruSeq Adapter, Index 27 (97% over 37bp)
GCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGCCGTCTTCTG30.11732499022291748TruSeq Adapter, Index 18 (96% over 27bp)
AGATCGGAAGAGCCACGTCTGAACTCCAGTCACATCCTCTATCTCGTATG30.11732499022291748TruSeq Adapter, Index 1 (95% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATTTCGTAT30.11732499022291748TruSeq Adapter, Index 18 (97% over 37bp)
TGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTAT30.11732499022291748TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACAGGTCTGAACTCCAGTCACATCCTCTATCTCGTATG30.11732499022291748TruSeq Adapter, Index 1 (97% over 36bp)
TGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTA30.11732499022291748TruSeq Adapter, Index 18 (97% over 37bp)
TGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCC30.11732499022291748TruSeq Adapter, Index 18 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)