FastQCFastQC Report
Mon 28 Oct 2024
000000000-LMV92_l01_n01_HRG_01EB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LMV92_l01_n01_HRG_01EB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG24411.657907310081223TruSeq Adapter, Index 20 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTAT894.252269469660774TruSeq Adapter, Index 20 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCG291.385570950788342TruSeq Adapter, Index 20 (97% over 35bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTC190.9077878643096035TruSeq Adapter, Index 20 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGC150.716674629718108TruSeq Adapter, Index 20 (97% over 37bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCAT140.6688963210702341TruSeq Adapter, Index 20 (97% over 38bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATA90.43000477783086477TruSeq Adapter, Index 20 (97% over 38bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATA80.3822264691829909TruSeq Adapter, Index 20 (97% over 38bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTC80.3822264691829909TruSeq Adapter, Index 20 (97% over 38bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGT60.2866698518872432TruSeq Adapter, Index 20 (97% over 34bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCC50.23889154323936934TruSeq Adapter, Index 20 (97% over 36bp)
GATCGGAAGAGGACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG40.19111323459149546TruSeq Adapter, Index 21 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)