FastQCFastQC Report
Fri 8 Nov 2024
000000000-LMT66_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LMT66_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578790
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC85929424.010741060526044TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGC140180.39169663489615203TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACCGATGTATCTCGTATGC103990.2905730707864949TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCCGAACTCCAGTCACCGATGTATCTCGTATGC67060.18738176869835893TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCGGAACTCCAGTCACCGATGTATCTCGTATGC64930.18143003640895386TruSeq Adapter, Index 2 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGT59240.1655308078987591TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGCATCTCGTATGC43680.12205242554047598TruSeq Adapter, Index 2 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)