FastQCFastQC Report
Fri 8 Nov 2024
000000000-LMT66_l01_n01_603.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LMT66_l01_n01_603.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186401
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCGGATCTCGTAT978188.244935734207912TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCGGATCTCGTA32260.27191480789378975TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCGGATATCGTAT25850.21788585815419914TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)