FastQCFastQC Report
Fri 8 Nov 2024
000000000-LMT66_l01_n01_213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LMT66_l01_n01_213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386332
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTACATATCTCGTAT15888811.461035307559806TruSeq Adapter, Index 11 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTACATATCTCGTA29660.2139458657810683TruSeq Adapter, Index 11 (97% over 41bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACGTCTACATATCTCGTAT15780.11382554828136406TruSeq Adapter, Index 18 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)