FastQCFastQC Report
Wed 17 Jul 2024
000000000-LL9B8_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LL9B8_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10232349
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC317586931.03753595582012Illumina Single End PCR Primer 1 (100% over 8bp)
CTAGTATG3276703.2022949959974976No Hit
ACGCGTGA2918242.8519746541092372No Hit
CGTTACTA2890462.8248254628531533No Hit
TCTACACT2754962.692402301758863Illumina Single End PCR Primer 1 (100% over 8bp)
GATAGCGT2688002.6269627824461423No Hit
GATCGTGT2651142.590939773457688No Hit
GTCAGATA2630352.5706218581872062No Hit
GTCTAGTG2616642.557223175245489No Hit
CTACTATA2394312.339941688853654No Hit
TGCGTCAC2154072.1051568901725304No Hit
GGATATCT2119082.0709614185364473No Hit
AAGCAGCA2119022.070902780974339No Hit
CGATCTAC2117742.069651846316032No Hit
TCGACGAG2023991.9780306555220117No Hit
GACACTGA1946081.901889781124549No Hit
CTAGAGCT1885461.8426462975412585No Hit
GACACCGT1835891.7942018983128898No Hit
TGCGTACG1784331.7438126866079335No Hit
ACTATCTG1753661.713839119443639No Hit
CTGCGTGT1746821.7071544373633074No Hit
TCATCGAG1696391.6578695664113878No Hit
GCTCTAGT1661121.6234004528188004No Hit
AGAGTCAC1648421.6109888355059037No Hit
ATCGTACG1634411.5972969647536455RNA PCR Primer, Index 22 (100% over 8bp)
ACGTCTCG1629251.5922541344123426No Hit
TACGAGAC1565221.5296780827159042TruSeq Adapter, Index 14 (100% over 8bp)
TAGTGTAG1553841.5185564917693875No Hit
CGTGAGTG1507631.4733957960190764No Hit
TAGCGAGT1461021.427844183188044No Hit
ACGACGTG1439351.4066662503399756No Hit
CGTCGCTA1302791.2732071589817744No Hit
ATATACAC1005880.9830391828894811No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)