FastQCFastQC Report
Wed 17 Jul 2024
000000000-LL9B8_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LL9B8_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10232349
Sequences flagged as poor quality0
Sequence length8
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTACGAC2844982.780378190775158No Hit
ACGTGCGC2131462.083060302184767No Hit
AGCATACC2126042.0777633757409952No Hit
ATAGCGCT2112152.0641887801129535No Hit
TAGTCTCC1994331.9490441539865382No Hit
ACTCACTG1932281.8884031418396694No Hit
TCAGTCTA1916511.8729912359322378No Hit
GACATAGT1887761.8448940707554053No Hit
ACGCTACT1884981.8421771970443934No Hit
TAGCAGCT1879821.8371343667030904No Hit
ATGAGCTC1741491.701945467262698No Hit
CGTCATAC1711481.6726169132815933No Hit
ATAGTACC1691301.6528951465592114No Hit
CTCTAGAG1669141.6312383402872597No Hit
CGAAGTAT1657511.619872426165292No Hit
CGAGCTAG1602611.5662190568363141No Hit
CGAGAGTT1593201.5570227325123487TruSeq Adapter, Index 13 (100% over 8bp)
CGAGCGAC1592761.5565927237235555No Hit
CATCGTGA1547341.5122040892076687No Hit
CGTAGCGA1468651.4353009265028No Hit
TCTAGACT1464931.4316653976520934No Hit
TCTCTATG1450071.4171428281033027ABI Solid3 Adapter B (100% over 8bp)
GTCTGCTA1443861.4110738404251066No Hit
GTCTATGA1424891.3925345978719061No Hit
TGAGTACG1414451.382331662065084No Hit
TCCTCATG1357961.3271243973402391No Hit
CAGTAGGT1341901.311429076549285No Hit
TATAGCGA1313051.283234182102272No Hit
AACGCTGA1243901.2156543917726028No Hit
CTCGACTT1185841.1589127775059276No Hit
GTAACGAG1176111.1494037195173854No Hit
GATCTACG1109441.0842476150881875No Hit
GAGCTCGA1104731.079644566462696No Hit
TGCTCGTA1053081.029167398414577No Hit
GCGTATAC1021600.9984022241618224No Hit
CTGCGTAG959150.9373702949342326No Hit
CTACGACC947190.9256818742206703No Hit
TAACGTCC890210.8699957360719421No Hit
TGCGTCAA870380.8506160217951909No Hit
ACTGTGTA842070.8229488654071514No Hit
ACGTACGT758450.7412276496823945No Hit
TACTAGGT660580.6455800129569467No Hit
GTTACAGC655900.6410062831125092No Hit
CTATCGTG584080.5708171212690263No Hit
CGCGATAT575120.5620605786608726No Hit
GAGACTTA531070.5190108351464556No Hit
GCGATACG518060.5062962570960001No Hit
AGTCGCAG425500.4158380446171256No Hit
ACGTATAC160940.15728548742815554No Hit
ACGATACG122390.11961085377365452No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)