FastQCFastQC Report
Tue 18 Jun 2024
000000000-LJVBV_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LJVBV_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10537851
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC245276323.275741894623486Illumina Single End PCR Primer 1 (100% over 8bp)
CTAGTATG4346254.1244177774007245No Hit
ACGCGTGA4164933.952352334456048No Hit
TCTACACT3941443.7402692446495966Illumina Single End PCR Primer 1 (100% over 8bp)
GTCTAGTG3740143.5492435791699846No Hit
GATAGCGT3519073.339456972773671No Hit
CGATCTAC2795442.6527609851382414No Hit
AAGCAGCA2759762.618902089240017No Hit
TGCGTCAC2694932.5573810068105916No Hit
GTCAGATA2154202.0442498190570353No Hit
GGATATCT1922341.8242239333237869No Hit
CGTTACTA1799651.7077960202701672No Hit
GACACCGT1766781.6766037022159452No Hit
TCATCGAG1653741.56933325400027No Hit
ACTATCTG1653571.5691719307855085No Hit
TCGACGAG1514541.4372380099130269No Hit
GATCGTGT1395391.3241694155667982No Hit
CTGCGTGT1384271.3136169794012083No Hit
TAGCGAGT1334151.2660550998491058No Hit
ATCGTACG1323871.256299790156456RNA PCR Primer, Index 22 (100% over 8bp)
ACGTCTCG1322251.2547624748157855No Hit
CGTGAGTG1264951.2003870618402177No Hit
TACGAGAC1119701.062550609227631TruSeq Adapter, Index 14 (100% over 8bp)
CTACTATA1105351.0489330319815682No Hit
AGAGTCAC936270.8884828604997357No Hit
TATAACCT900380.8544246829832761No Hit
GGAAGCAG757100.7184576817417517No Hit
CAGACAAG729380.6921525081347232No Hit
CCTTGAAG674460.6400356201658194No Hit
TTGGTGAG639590.6069453819379302No Hit
AAGGATGA611090.5799000194631714No Hit
CAGCGTAA606160.5752216462350814No Hit
CTAGAGCT605320.5744245197621413No Hit
TTGGAGAG588900.5588425951363328No Hit
CACTGAGA587730.5577323118347375No Hit
TCCAACAG568320.5393129965492964No Hit
GACACTGA567870.5388859645102213No Hit
CGCGGTTC529220.5022086571541009No Hit
TCGTGGAA524910.49811863917984794No Hit
CGTCGCTA480250.4557380817018574No Hit
ACCAAGGA463650.4399853442604189No Hit
GCTCTAGT455310.4320710171362263No Hit
GCGACTAA453210.4300782009538757No Hit
AATGGCGA448820.42591226617267597No Hit
AGGAACTG426590.4048168834423641No Hit
AGCACCAA424570.40289998406696015No Hit
ATATACAC412080.39104747258240796No Hit
ACGACGTG408860.3879918211028036No Hit
CGGAAGAA406730.38597053611784793No Hit
AGTTCAGG406500.3857522752978762No Hit
GGTTGCAA400160.3797358683473509No Hit
TGAGGCAT392040.3720303124422617No Hit
GTGCTGAA381190.3617340955001167No Hit
TGTAGGTG380220.36081360421588804No Hit
TTGTGAGC361890.3434191658242274No Hit
ATGTCGTG343260.3257400394065166No Hit
TCGGTTCA341920.32446843289015953No Hit
CTGTGCTA314910.29883702094478276No Hit
GCAGAGAA305660.2900591401415715No Hit
AGCATTGG300870.2855136213256384No Hit
AGGTTGCA293960.2789563071256179No Hit
CCGTAACA282200.2677965365044543No Hit
TCTCTGGA280400.2660884083481537No Hit
TTGCCTGA267210.253571624802818No Hit
CTAGGAAC264610.25110432857705045No Hit
TGCGTGAA260930.2476121554575027No Hit
GCACTAAC257400.2442623263509799No Hit
GACAATGC245490.2329602117167912No Hit
AGACGGAA234930.2229391931998279TruSeq Adapter, Index 14 (100% over 8bp)
GTGAACCA226660.2150912932817137No Hit
GGCCATAA217880.2067594237193143No Hit
AAGGTACC208780.19812388692912816No Hit
AGCTAGAG197350.1872772731366196No Hit
CGGTTAAC166670.1581631776725634No Hit
ATGGATGC166450.15795440645346No Hit
CATAGACC138270.13121271120648792No Hit
CTGACTTC131100.1244086673838907No Hit
TTACCGAC125850.11942662692801408No Hit
AGTCCTTC119860.11374235600788055No Hit
TGTTAGCC109840.10423377593780743No Hit
ATAGCCTC109670.10407245272304573No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)