FastQCFastQC Report
Tue 18 Jun 2024
000000000-LJVBV_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LJVBV_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10537851
Sequences flagged as poor quality0
Sequence length8
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACATAGT4432094.205876511254524No Hit
ACTACGAC3773693.5810811900832533No Hit
ACTCACTG3267903.1011066677636645No Hit
GTCTGCTA3263573.0969976705876747No Hit
ACGCTACT2932362.7826926002275036No Hit
CTGCGTAG2880412.733394123716496No Hit
CGAGAGTT2846022.700759386330287TruSeq Adapter, Index 13 (100% over 8bp)
TAGTCTCC2677612.540945018106633No Hit
TGAGTACG2576672.4451569869416447No Hit
TATAGCGA2415712.2924123713649016No Hit
CGAGCGAC2322382.2038459264607178No Hit
GTCTATGA2209262.096499561438096No Hit
ACAGTGGT1851101.7566200167377581No Hit
CATTACGG1656331.5717910606251693No Hit
CGTGTACA1516561.4391549092884308No Hit
GATTCGGA1396971.3256687725039953No Hit
CATCTTCG1302831.2363336699294762No Hit
AACCTCCA1253541.1895594272494459No Hit
GGTTGCAA1158151.0990381245663845No Hit
GAACCACA1124791.067380816069614No Hit
GCATAAGG1028030.9755594380675908No Hit
CTCTCGTC946280.8979819509689405No Hit
AGCATACC937210.8893748829813592No Hit
AACGCTGA921870.8748178352493311No Hit
GCGACTAA879900.8349899804049231No Hit
TGCTCGTA828330.7860521087269121No Hit
CACTAGCT811600.7701760064741853Illumina PCR Primer Index 10 (100% over 8bp)
TTGGACTC793510.7530093185033647No Hit
TCAGTCTA750410.7121091387608346No Hit
ACCGGTAA734190.696717006152393No Hit
CTACGACC730220.6929496346076633No Hit
TAACGTCC708830.6726513783502917No Hit
TGCGTCAA686560.6515180372165065No Hit
GACCTGAA666760.6327286274972003No Hit
ATAGCGCT664660.6307358113148497No Hit
ATGAGCTC642980.6101623566322963No Hit
CCAAGTCT637540.6050000137599212No Hit
ACTGTGTA633490.601156725408245No Hit
GCGTATAC626300.5943337023839111No Hit
GAGCTCGA583900.5540977947021646No Hit
ACGTACGT582670.5529305737953593No Hit
TCTCTACT578010.5485084197907144No Hit
TAGCAGCT544840.5170314137104425No Hit
CGAAGTAT537290.5098667650548484No Hit
TCCTCATG534050.5067921343735075No Hit
CAGTAGGT533880.5066308111587458No Hit
TACTAGGT517050.4906598128973355No Hit
GTTACAGC511980.48584858525708896No Hit
CTCTAGAG497320.4719368303841077No Hit
CGAGCTAG492000.4668883627221528No Hit
CGTCATAC467900.4440184246294619No Hit
CTATCGTG449250.4263203190100145No Hit
CTCGACTT445370.42263835387309995No Hit
CGTAGCGA428750.40686663722992483No Hit
CGCGATAT427620.40579431233180274No Hit
GAGACTTA415350.39415057206635395No Hit
CATCGTGA414570.3934103831986237No Hit
GCGATACG406650.38589461931090124No Hit
TCTCTATG384740.3651029038083761ABI Solid3 Adapter B (100% over 8bp)
GTAACGAG370790.3518649105970468No Hit
ACGTGCGC353590.3355427971035081No Hit
TCTAGACT335340.31822427551879406No Hit
AGTCGCAG318200.30195909963046547No Hit
AACATAGT303350.2878670423409858No Hit
GATCTACG261330.24799173949223613No Hit
ATAGTACC240980.22868040172517148No Hit
AGTTGCAA236730.2246473213561285No Hit
ATCTGCTA216340.20529802518559048No Hit
ACATAAGG159470.15133066504736117No Hit
ACGACTAA148950.14134760493387125No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)