FastQCFastQC Report
Mon 3 Jun 2024
000000000-LJMMB_l01_n02_BS_CR_51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LJMMB_l01_n02_BS_CR_51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4871774
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAAG242210.49717002471789534ABI Solid3 Adapter B (100% over 41bp)
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAAG242030.496800549450775ABI Solid3 Adapter B (100% over 41bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACCAGGTGTGTAGATCTC104020.2135156515881073Illumina Single End PCR Primer 1 (96% over 33bp)
CCATCTCATCCCTGCGTGTCTCCGACTCAGAGATCGGAAGAGCGTCGTGTA56280.11552260018629765Illumina Single End PCR Primer 1 (100% over 21bp)
CTGAGTCGGAGACACGCAGGGATGAGATGGAGATCGGAAGAGCGTCGTGTA53430.1096725751235587Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)