FastQCFastQC Report
Tue 22 Oct 2024
000000000-LJM9J_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LJM9J_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604632
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC42157916.185741402240318Illumina Single End PCR Primer 1 (100% over 8bp)
TCGACGAG1513955.812529370751799No Hit
GTCAGATA1442445.537980029424502No Hit
CTACTATA1390585.338873207424312No Hit
ACGTCTCG1360055.221658952205148No Hit
CGTTACTA1353055.194783754480479No Hit
TACGAGAC1339705.143528913105575TruSeq Adapter, Index 14 (100% over 8bp)
GATCGTGT1311985.037103130115886No Hit
AGAGTCAC1225134.703658712631957No Hit
GACACCGT724992.783464228343966No Hit
TCATCGAG706282.711630664139886No Hit
CTGCGTGT702322.696426980855645No Hit
TAGCGAGT698502.681760801525897No Hit
CGTGAGTG651802.502464839562748No Hit
ACTATCTG597492.293951698358924No Hit
GGATATCT562092.158039984151312No Hit
ATCGTACG550952.1152700266294815RNA PCR Primer, Index 22 (100% over 8bp)
TAGTGTAG471841.8115418992011154No Hit
GACACTGA410391.5756160563181285No Hit
CGTCGCTA401511.5415229483474058No Hit
GCTCTAGT394031.5128048799216165No Hit
TGCGTACG361761.3889102184108926No Hit
CTAGAGCT358271.375511012688165No Hit
ATATACAC281191.0795766925999528No Hit
ACGACGTG227360.8729064220972483No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)