FastQCFastQC Report
Tue 22 Oct 2024
000000000-LJM9J_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LJM9J_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604632
Sequences flagged as poor quality0
Sequence length8
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGTACC1175514.513151953903661No Hit
CTCGACTT937973.6011613156868223No Hit
CGAAGTAT934953.589566587525608No Hit
TAGCAGCT930983.5743245111017603No Hit
GATCTACG928423.564495867362453No Hit
TCTCTATG774422.9732415174197353ABI Solid3 Adapter B (100% over 8bp)
GCGTATAC740032.8412075103123975No Hit
ACGTGCGC727622.79356162406052No Hit
TGCTCGTA661092.5381320662573446No Hit
GTAACGAG604262.319943853872639No Hit
AGCATACC536042.058025855475937No Hit
ATGAGCTC516191.9818154733566968No Hit
CGTCATAC513121.9700287794974494No Hit
TCTAGACT490631.8836826085220484No Hit
CGAGCTAG483841.8576136667291194No Hit
ATAGCGCT477951.8350001075007907No Hit
CTCTAGAG461811.7730335801756254No Hit
TCCTCATG457171.7552191633981307No Hit
TCAGTCTA449341.7251573350861082No Hit
TAGTCTCC446181.7130251029704007No Hit
ACTCACTG405001.5549221540701335No Hit
ACGCTACT387061.4860448616157675No Hit
CGAGAGTT380851.4622027219200255TruSeq Adapter, Index 13 (100% over 8bp)
ACTACGAC380681.4615500385467122No Hit
GACATAGT371411.425959598131329No Hit
CGAGCGAC350841.346984909960409No Hit
CATCGTGA337141.294386308699271No Hit
AACGCTGA336311.2911996781119175No Hit
TGAGTACG332981.2784147626228963No Hit
CTGCGTAG319141.2252786574072652No Hit
GTCTGCTA313671.2042776100424166No Hit
CAGTAGGT276021.0597274394233045No Hit
CGTAGCGA232480.8925637095758634No Hit
AGTCGCAG194240.7457483437199574No Hit
TACTAGGT182720.7015194468930736No Hit
ACGTACGT179410.6888113176832659No Hit
CTATCGTG179320.6884657794268059No Hit
CTACGACC178370.6848184311641722No Hit
GTTACAGC176810.6788291013855317No Hit
TAACGTCC164390.6311448219940475No Hit
CGCGATAT159830.6136375503334061No Hit
GCGATACG146280.5616148461663683No Hit
GAGACTTA132920.5103216116518572No Hit
ACGTATAC100870.3872715992124799No Hit
AATCTACG67390.2587313678093489No Hit
ATAACGAG52820.20279256340243074No Hit
GTCTATGA50830.19515232862070342No Hit
TATAGCGA50150.19254159512744987No Hit
ACTGTGTA44800.17200126543788144No Hit
GAGCTCGA29030.1114552842781629No Hit
AACATAGT26170.1004748463506553No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)